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Titolo:
HOXB7 CONSTITUTIVELY ACTIVATES BASIC FIBROBLAST GROWTH-FACTOR IN MELANOMAS
Autore:
CARE A; SILVANI A; MECCIA E; MATTIA G; STOPPACCIARO A; PARMIANI G; PESCHLE C; COLOMBO MP;
Indirizzi:
IST NAZL TUMORI,VIA VENEZIAN 1 I-20133 MILAN ITALY IST SUPER SANITA,DEPT HEMATOL & ONCOL I-00161 ROME ITALY IST NAZL STUDIO & CURA TUMORI,DIV EXPT ONCOL D I-20133 MILAN ITALY UNIV ROMA LA SAPIENZA,DEPT EXPT MED & PATHOL I-00100 ROME ITALY THOMAS JEFFERSON UNIV PHILADELPHIA PA 19107
Titolo Testata:
Molecular and cellular biology
fascicolo: 9, volume: 16, anno: 1996,
pagine: 4842 - 4851
SICI:
0270-7306(1996)16:9<4842:HCABFG>2.0.ZU;2-I
Fonte:
ISI
Lingua:
ENG
Soggetto:
CELL-ADHESION MOLECULE; HOMEOBOX GENE; IN-VITRO; MALIGNANT-MELANOMA; HUMAN MELANOCYTES; TRANSGENIC MICE; NUDE-MICE; EXPRESSION; LEUKEMIA; PROLIFERATION;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Science Citation Index Expanded
Citazioni:
55
Recensione:
Indirizzi per estratti:
Citazione:
A. Care et al., "HOXB7 CONSTITUTIVELY ACTIVATES BASIC FIBROBLAST GROWTH-FACTOR IN MELANOMAS", Molecular and cellular biology, 16(9), 1996, pp. 4842-4851

Abstract

Homeobox (HOX) genes control axial specification during mammalian development and also regulate skin morphogenesis. Although selected HOX genes are variably expressed in leukemias and kidney and colon cancer cell lines, their relationship with the neoplastic phenotype remains unclear. In both normal development and neoplastic transformation, HOX target genes are largely unknown. We investigated the expression and function of HOXB cluster genes in human melanoma. The HOXB7 gene was constitutively expressed in all 25 melanoma cell lines and analyzed underboth normal and serum-starved conditions, as well as in in vivo primary and metastatic melanoma cells; conversely, HOXB7 was expressed in proliferating but not quiescent normal melanocytes. Treatment of melanoma cell lines with antisense oligomers targeting HOXB7 mRNA markedly inhibited cell proliferation and specifically abolished expression of basic fibroblast growth factor (bFGF) mRNA. Band shift and cotransfection experiments showed that HOXB7 directly transactivates the bFGF genethrough one out of five putative homeodomain binding sites present inits promoter. These novel findings indicate a key role for constitutive HOXB7 expression in melanoma cell proliferation via bFGF. The results also raise the possibility that growth factor genes are critical HOX target genes in other developmental and/or neoplastic cell systems.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 01/10/20 alle ore 07:40:54