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Titolo:
CANINE PROSOMATOSTATIN - ISOLATION OF A CDNA, REGULATION OF GENE-EXPRESSION, AND CHARACTERIZATION OF POSTTRANSLATIONAL PROCESSING INTERMEDIATES
Autore:
DICKINSON CJ; DELVALLE J; TODISCO A; GANTZ I; TONG L; FINNISS S; YAMADA T;
Indirizzi:
UNIV MICHIGAN,MED CTR,DEPT PEDIAT,1150 W MED CTR DR,A420 MSRB 1 ANN ARBOR MI 48109 UNIV MICHIGAN,MED CTR,DEPT INTERNAL MED ANN ARBOR MI 48109 UNIV MICHIGAN,MED CTR,DEPT SURG ANN ARBOR MI 48109 UNIV MICHIGAN,MED CTR,DEPT PHYSIOL ANN ARBOR MI 48109
Titolo Testata:
Regulatory peptides
fascicolo: 3, volume: 67, anno: 1996,
pagine: 145 - 152
SICI:
0167-0115(1996)67:3<145:CP-IOA>2.0.ZU;2-R
Fonte:
ISI
Lingua:
ENG
Soggetto:
SOMATOSTATIN-LIKE IMMUNOREACTIVITY; PROPEPTIDE CONVERTING ENZYME; FUNDIC MUCOSAL CELLS; SECRETORY PATHWAYS; MONOBASIC SITES; MULTIPLE FORMS; RAT; PANCREAS; RELEASE; PEPTIDES;
Keywords:
PROSOMATOSTATIN; GENE EXPRESSION; CDNA; REGULATION; DOG;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Science Citation Index Expanded
Citazioni:
41
Recensione:
Indirizzi per estratti:
Citazione:
C.J. Dickinson et al., "CANINE PROSOMATOSTATIN - ISOLATION OF A CDNA, REGULATION OF GENE-EXPRESSION, AND CHARACTERIZATION OF POSTTRANSLATIONAL PROCESSING INTERMEDIATES", Regulatory peptides, 67(3), 1996, pp. 145-152

Abstract

Somatostatin is a tetradecapeptide (SS-14) initially isolated from the hypothalamus that is also found in D cells of the stomach and pancreas where it exerts an inhibitory action on a variety of gastrointestinal functions. Since many of concepts important to an understanding of gastrointestinal physiology are derived from experiments in the dog weexamined somatostatin gene expression and post-translational processing in the canine fundus, antrum and pancreas. The canine somatostatin cDNA which is highly homologous to other known mammalian somatostatinswas used to examine somatostatin expression in isolated canine fundicD-cells. Somatostatin expression induced by cholecystokinin (10(-8) M) was inhibited by the somatostatin analog, octreotide (10(-7) M). To examine somatostatin processing in the canine gut we noted that synthesis of SS-14 and somatostatin octacosapeptide (SS-28) involves endoproteolytic cleavage of prosomatostatin (proSS) at both paired and singlebasic amino-acid residues, respectively. Antisera capable of recognizing the amino-terminal residues of SS-28, SS-28((1-14)) and SS-28((1-12)) were characterized and identified concentrations of SS-28((1-12)) but not SS-28((1-14)) in the fundus, antrum and pancreas equivalent tothose of SS-14. Since previous biosynthetic studies in canine fundic D-cells showed that SS-14 was synthesized without the appearance of a SS-28 intermediate, we hypothesize that proSS is sequentially cleaved at a dibasic site to produce SS-14 followed by monobasic cleavage thatresults in the formation of SS-28((1-12)). Furthermore, equivalent amounts of SS-14 and SS-28((1-12)) were co-released from canine fundic D-cells by CCK (10(-8) M) suggesting that the generation of these products occurs within the same regulated pathway of secretion.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 27/09/20 alle ore 04:57:13