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Titolo:
IDENTIFICATION OF THE TRANSCRIPTION FACTORS NF-YA AND NF-YB AS FACTOR-A AND FACTOR-B THAT BOUND TO THE PROMOTER OF THE MAJOR HISTOCOMPATIBILITY COMPLEX CLASS-II GENE-I-A-BETA
Autore:
CELADA A; MCKERCHER SR; MAKI RA;
Indirizzi:
UNIV BARCELONA,FAC BIOL,DEPT FISIOL IMMUNOL,CAMPUS BELLVITGE E-08028 BARCELONA SPAIN UNIV BARCELONA,FAC BIOL,DEPT FISIOL IMMUNOL E-08028 BARCELONA SPAIN UNIV BARCELONA,FUNDACIO AUGUST PI & SUNYER E-08028 BARCELONA SPAIN LA JOLLA CANC RES FDN LA JOLLA CA 92037
Titolo Testata:
Biochemical journal
, volume: 317, anno: 1996,
parte:, 3
pagine: 771 - 777
SICI:
0264-6021(1996)317:<771:IOTTFN>2.0.ZU;2-G
Fonte:
ISI
Lingua:
ENG
Soggetto:
MHC CLASS-II; BARE LYMPHOCYTE SYNDROME; CELL-SPECIFIC ENHANCER; BOX-BINDING-PROTEINS; TRANS-ACTING FACTOR; DR-ALPHA GENE; DNA-BINDING; NUCLEAR FACTORS; HLA-DR; UPSTREAM ELEMENTS;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Citazioni:
51
Recensione:
Indirizzi per estratti:
Citazione:
A. Celada et al., "IDENTIFICATION OF THE TRANSCRIPTION FACTORS NF-YA AND NF-YB AS FACTOR-A AND FACTOR-B THAT BOUND TO THE PROMOTER OF THE MAJOR HISTOCOMPATIBILITY COMPLEX CLASS-II GENE-I-A-BETA", Biochemical journal, 317, 1996, pp. 771-777

Abstract

The Y box is a conserved sequence in the promoter of major histocompatibility complex (MHC) class II genes, which contains a CCAAT sequence(CCAAT box). Previously, we partially purified the DNA-binding protein that recognizes the Y box of the I-A beta gene and showed that it consisted of two components (factors A and B) both of which were necessary for optimal DNA binding. The genes for the heteromeric protein NF-Y(NF-YA and NF-YB), which binds to the I-E alpha Y box have been cloned. We subsequently isolated the genes for NF-YA and NF-YB using oligonucleotides designed from the published sequences. NF-YA and NF-YB weretested for binding to the I-A beta and I-E alpha Y boxes. While neither NF-YA or NF-YB alone bound to the Y box, when the components were mixed the complex bound to the I-A beta Y box with high affinity. Moreover, NF-YA and NF-YB could be complemented for binding to DNA by factor B or factor A, respectively. These results suggest that the active binding protein is NF-YA in factor A extracts and NF-MB in factor B extracts. Finally, antibodies against NF-YA and NF-YB were shown to induce a supershift when nuclear extracts were added to the double-strandedoligodeoxynucleotide covering the Y box of the I-A beta gene. Antisense expression constructs of both NF-YA and NF-YB were made and their effect on expression from the I-A beta promoter was tested. Either antisense construction, when transfected into cells, lowered the expression of a reporter gene linked to the I-A beta promoter. This study provides direct evidence of the identification of NF-YA and NF-YB as the previously described factors A and B. Moreover, these results strongly implicate NF-Y in the expression of the MHC class II gene I-A beta.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 14/07/20 alle ore 18:35:46