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Titolo:
DESMOPLAKIN EXPRESSION AND ORGANIZATION AT HUMAN UMBILICAL VEIN ENDOTHELIAL CELL-TO-CELL JUNCTIONS
Autore:
VALIRON O; CHEVRIER V; USSON Y; BREVIARIO F; JOB D; DEJANA E;
Indirizzi:
CEA,HEMATOL LAB,INSERM U217 GRENOBLE 9 FRANCE CEN GRENOBLE,DBMS,LAB CYTOSQUELETTE,INSERM U366 GRENOBLE 9 FRANCE UNIV GRENOBLE 1,INST ALBERT BONNIOT,DYOGEN,UPRES 950456 F-38041 GRENOBLE FRANCE IST RIC FARMACOL MARIO NEGRI MILAN ITALY
Titolo Testata:
Journal of Cell Science
, volume: 109, anno: 1996,
parte:, 8
pagine: 2141 - 2149
SICI:
0021-9533(1996)109:<2141:DEAOAH>2.0.ZU;2-8
Fonte:
ISI
Lingua:
ENG
Soggetto:
INTERMEDIATE FILAMENT NETWORKS; KIDNEY EPITHELIAL-CELLS; COMPLEXUS ADHAERENTES; SPATIAL REGULATION; DESMOSOMAL PLAQUE; BETA-CATENIN; CADHERINS; ADHESION; DIFFERENTIATION; STABILIZATION;
Keywords:
DESMOPLAKIN; ENDOTHELIUM; ADHERENCE JUNCTION; CADHERIN; CATENIN;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Citazioni:
43
Recensione:
Indirizzi per estratti:
Citazione:
O. Valiron et al., "DESMOPLAKIN EXPRESSION AND ORGANIZATION AT HUMAN UMBILICAL VEIN ENDOTHELIAL CELL-TO-CELL JUNCTIONS", Journal of Cell Science, 109, 1996, pp. 2141-2149

Abstract

Desmoplakin is an intracellular component of desmosomes which plays arole in the anchorage of intermediate filaments to these structures. We report here that, despite the absence of desmosomes, cultured endothelial cells from human umbilical vein express desmoplakin I and II both at mRNA and protein level, Desmoplakin I/II are found only in the detergent insoluble fraction suggesting that most of the protein is linked to the cytoskeleton, Desmoplakin I/II could be detected by westernblot only in long confluent cells even if desmoplakin mRNA levels areunchanged by cell confluency. This suggests that desmoplakin might bestabilized at protein level by its association with junctional components, Immunofluorescence confocal microscopy showed that desmoplakin codistributes with VE-cadherin and plakoglobin along the lateral cell membrane, In contrast, desmoplakin localization was distinct from that of PECAM, an endothelial specific junctional protein localized outsideadherence junctions, Endothelial cells do not have keratins but they express vimentin, In confluent cells vimentin forms peripheral filaments which attach to the cell membrane in areas at desmoplakin localization, These data suggest that desmoplakin may participate in the molecular organization of interendothelial junctions by interacting with VE-cadherin and promoting vimentin anchorage, This new type of intercellular junction seems to correspond to the 'complexus adhaerentes' described in vivo in lymphatic endothelium.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 10/07/20 alle ore 14:59:04