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Titolo:
HIGH-LEVEL IL-12 PRODUCTION BY MURINE DENDRITIC CELLS - UP-REGULATIONVIA MHC CLASS-II AND CD40 MOLECULES AND DOWN-REGULATION BY IL-4 AND IL-10
Autore:
KOCH F; STANZL U; JENNEWEIN P; JANKE K; HEUFLER C; KAMPGEN E; ROMANI N; SCHULER G;
Indirizzi:
UNIV ERLANGEN NURNBERG,DEPT DERMATOL,HARTMANNSTR 14 D-91052 ERLANGEN GERMANY UNIV ERLANGEN NURNBERG,DEPT DERMATOL D-91052 ERLANGEN GERMANY INNSBRUCK UNIV,DEPT DERMATOL A-6020 INNSBRUCK AUSTRIA UNIV WURZBURG D-8700 WURZBURG GERMANY
Titolo Testata:
The Journal of experimental medicine
fascicolo: 2, volume: 184, anno: 1996,
pagine: 741 - 746
SICI:
0022-1007(1996)184:2<741:HIPBMD>2.0.ZU;2-A
Fonte:
ISI
Lingua:
ENG
Soggetto:
T-CELLS; IN-VIVO; INTERLEUKIN-12; ANTIGEN; INDUCTION; CYTOKINES; LIGAND; SELF;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Science Citation Index Expanded
Citazioni:
21
Recensione:
Indirizzi per estratti:
Citazione:
F. Koch et al., "HIGH-LEVEL IL-12 PRODUCTION BY MURINE DENDRITIC CELLS - UP-REGULATIONVIA MHC CLASS-II AND CD40 MOLECULES AND DOWN-REGULATION BY IL-4 AND IL-10", The Journal of experimental medicine, 184(2), 1996, pp. 741-746

Abstract

We have shown previously that dendritic cells (DC) produce IL-12 uponinteraction with CD4+ T cells. Here we ask how this IL-12 production is induced and regulated. Quantitative PCR and in situ hybridization for IL-12 p40 and an ELISA specific for the F70 heterodimer were used to determine IL-12 production. We demonstrate that ligation of either CD40 or MHC class II molecules independently trigger IL-12 production ill DC, and that IL-12 production is downregulated by IL-4 and IL-10. The levels of bioactive IL-12. that can be released by triggering with all anti-CD40 mAb or with a T cell hybridoma are high (range 260-4700 pg/ml from 1 x 10(6) DC in 72 h). The CD40-mediated pathway indicates that IL-12. production is induced in DC upon interaction with activated, CD40 ligand-expressing helper T cells, even in the absence of cognate antigen recognition. Side-by-side comparison of IL-12 production, and blocking experiments employing an anti-CD40 ligand mAb, suggest that the CD40-mediated pathway is quantitatively more significant than induction via the MHC class II molecule. The importance of the CD40/CD40ligand interaction for IL-12 induction in DC likely contributes to the recent finding that mice lacking the CD40 ligand are impaired in mounting Th1 type cell-mediated immune responses.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 27/11/20 alle ore 22:13:41