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Titolo:
CD27 COOPERATES WITH THE PRE-T CELL-RECEPTOR IN THE REGULATION OF MURINE T-CELL DEVELOPMENT
Autore:
GRAVESTEIN LA; VANEWIJK W; OSSENDORP F; BORST J;
Indirizzi:
NETHERLANDS CANC INST,DIV CELLULAR BIOCHEM,PLESMANLAAN 121 NL-1066 CXAMSTERDAM NETHERLANDS NETHERLANDS CANC INST,DIV CELLULAR BIOCHEM NL-1066 CX AMSTERDAM NETHERLANDS ERASMUS UNIV ROTTERDAM,DEPT IMMUNOL NL-3000 DR ROTTERDAM NETHERLANDS ACAD HOSP LEIDEN,DEPT IMMUNOHAEMATOL NL-2333 AA LEIDEN NETHERLANDS
Titolo Testata:
The Journal of experimental medicine
fascicolo: 2, volume: 184, anno: 1996,
pagine: 675 - 685
SICI:
0022-1007(1996)184:2<675:CCWTPC>2.0.ZU;2-Y
Fonte:
ISI
Lingua:
ENG
Soggetto:
GROWTH-FACTOR RECEPTOR; BETA-CHAIN; THYMOCYTE DIFFERENTIATION; TRANSGENIC MICE; CONTROL POINTS; LYMPHOCYTES-T; MUTANT MICE; ALPHA-BETA; EXPRESSION; LIGAND;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Science Citation Index Expanded
Citazioni:
45
Recensione:
Indirizzi per estratti:
Citazione:
L.A. Gravestein et al., "CD27 COOPERATES WITH THE PRE-T CELL-RECEPTOR IN THE REGULATION OF MURINE T-CELL DEVELOPMENT", The Journal of experimental medicine, 184(2), 1996, pp. 675-685

Abstract

CD27 is a lymphocyte-specific member of the TNF receptor family and has a TNF-related transmembrane ligand, CD70. The CD27/CD70 receptor-ligand pair cooperates with the TCR in the regulation of the peripheral T cell response. The study presented here reveals that CD27 may play asimilar role in thymic pre-T cell development. We have previously cloned the cDNA encoding murine CD27, prepared specific mAbs and observedthat murine CD27 is expressed on virtually all thymocytes, with the exception of a subpopulation of CD4(-)8(-) precursor T cells. It is shown here that induction of murine CD27 expression occurs at the transition from the CD4(-)8(-)25(+) to the CD4(-)8(-)25(-) precursor T cell stage and is regulated by the pre-TCR. Therefore, we investigated whether CD27 contributes to pre-TCR-mediated thymocyte development. Pre-TCRfunction was mimicked by the induction of CD3 signaling in thymocytesof recombination activating gene (RAG)-deficient mice. This ill vivo anti-CD3 epsilon mAb treatment induces an about fifty fold numerical expansion of CD4(-)8(-)25(+) thymocytes and their differentiation to the CD4(+)8(+)25(-) stage. Co-injection of anti-CD27 mAb inhibited the CD3-mediated. expansion and differentiation of the CD4(-)8(-)25(+) precursor population. Also, injection of anti-CD27 mAb in TCR alpha(-/-) mutant mice led to a reduction in the absolute number- of CD4(+)8(+)25(-) thymocytes. The present evidence that ill these in vivo systems, anti-CD27 mAb inhibits CD27-ligand interaction. Therefore, we conclude that CD27 may contribute to normal murine T cell development by synergizing with the pre-TCR-mediated signal.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 24/09/20 alle ore 03:56:35