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Titolo:
FOURIER-TRANSFORM INFRARED MICROSPECTROSCOPIC ANALYSIS IDENTIFIES ALTERATIONS IN MINERAL PROPERTIES IN BONES FROM MICE TRANSGENIC FOR TYPE-X COLLAGEN
Autore:
PASCHALIS EP; JACENKO O; OLSEN B; MENDELSOHN R; BOSKEY AL;
Indirizzi:
HOSP SPECIAL SURG,535 E 70TH ST NEW YORK NY 10021 HOSP SPECIAL SURG NEW YORK NY 10021 UNIV PENN,SCH VET MED PHILADELPHIA PA 19104 HARVARD UNIV,SCH MED BOSTON MA 00000 RUTGERS STATE UNIV,DEPT CHEM NEWARK NJ 07102
Titolo Testata:
Bone
fascicolo: 2, volume: 19, anno: 1996,
pagine: 151 - 156
SICI:
8756-3282(1996)19:2<151:FIMAIA>2.0.ZU;2-H
Fonte:
ISI
Lingua:
ENG
Soggetto:
SCHMID METAPHYSEAL CHONDRODYSPLASIA; FT-IR MICROSCOPY; HYPERTROPHIC CHONDROCYTES; CARTILAGE; EXPRESSION; MUTATION; DOMAIN; 1,25-DIHYDROXYVITAMIN-D3; RESOLUTION; CULTURES;
Keywords:
FTIR; FTIR MICROSPECTROSCOPY; COLLAGEN X; TRANSGENIC MICE; 2ND DERIVATIVE SPECTROSCOPY; ENDOCHONDRAL OSSIFICATION;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Citazioni:
29
Recensione:
Indirizzi per estratti:
Citazione:
E.P. Paschalis et al., "FOURIER-TRANSFORM INFRARED MICROSPECTROSCOPIC ANALYSIS IDENTIFIES ALTERATIONS IN MINERAL PROPERTIES IN BONES FROM MICE TRANSGENIC FOR TYPE-X COLLAGEN", Bone, 19(2), 1996, pp. 151-156

Abstract

Type X collagen has been implicated in the morphogenetic events of endochondral ossification (EO), including the calcification of hypertrophic cartilage and trabeculae prior to their replacement by bone and marrow, Recently, transgenic mice, which expressed a truncated collagen X protein, were reported to exhibit morphologic alterations in all tissues arising through EO. Specifically, the growth plates mere compressed within the zone of cartilage hypertrophy, and the number and size of calcified trabeculae were reduced, The condition in the mouse is comparable to Schmid metaphyseal chondrodysplasia in humans for which, todate, 20 defined type X collagen mutations have been reported, The transgenic mouse showed no alterations in mineralization by conventionalhistology, however, it did show a decrease in newly formed bony trabeculae, and a thinning of periosteal bones, Fourier transform infrared (FTIR) spectroscopy has previously been shown to provide quantitative and qualitative information about the relative amount of mineral and carbonate present, mineral composition, and crystal perfection, To determine whether the expression of abnormal collagen X molecules had an effect on mineral properties, the ''quality'' of mineral crystals was analyzed in thin sections of tibia from day 17 and day 25 genotypicallynegative (normal) and positive (mutant) mice from several independenttransgenic mouse lines showing varying degrees of the mutant phenotype, by means of Fourier transform infrared microscopic analysis (FTIRM). The results indicate definite differences between normal and transgenic mice calcified cartilage mineral, both in the amount present and the ''quality'' of the crystals, Calcified cartilage mineral from transgenic mice exhibited less crystallinity and higher acidic phosphate content than the corresponding mineral from normal specimens.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 16/07/20 alle ore 06:35:30