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Titolo:
GENETIC MANIPULATION OF ACID FORMATION PATHWAYS BY GENE INACTIVATION IN CLOSTRIDIUM-ACETOBUTYLICUM ATCC-824
Autore:
GREEN EM; BOYNTON ZL; HARRIS LM; RUDOLPH FB; PAPOUTSAKIS ET; BENNETT GN;
Indirizzi:
RICE UNIV,INST BIOSCI & BIOENGN,DEPT BIOCHEM & CELL BIOL,MS 140,6100 MAIN ST HOUSTON TX 77005 RICE UNIV,INST BIOSCI & BIOENGN,DEPT BIOCHEM & CELL BIOL HOUSTON TX 77005 NORTHWESTERN UNIV,DEPT CHEM ENGN EVANSTON IL 60208
Titolo Testata:
Microbiology
, volume: 142, anno: 1996,
parte:, 8
pagine: 2079 - 2086
SICI:
1350-0872(1996)142:<2079:GMOAFP>2.0.ZU;2-I
Fonte:
ISI
Lingua:
ENG
Soggetto:
BACILLUS-SUBTILIS; ESCHERICHIA-COLI; MOLECULAR CHARACTERIZATION; BUTANOL DEHYDROGENASE; BUTYRATE KINASE; CHROMOSOME; VECTORS; DNA; PH; PHOSPHOTRANSBUTYRYLASE;
Keywords:
CLOSTRIDIUM ACETOBUTYLICUM; SOLVENT BIOSYNTHESIS; ACID BIOSYNTHESIS; METABOLIC ENGINEERING; GENE INTEGRATION;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Citazioni:
36
Recensione:
Indirizzi per estratti:
Citazione:
E.M. Green et al., "GENETIC MANIPULATION OF ACID FORMATION PATHWAYS BY GENE INACTIVATION IN CLOSTRIDIUM-ACETOBUTYLICUM ATCC-824", Microbiology, 142, 1996, pp. 2079-2086

Abstract

Integrational plasmid technology has been used to disrupt metabolic pathways leading to acetate and butyrate formation in Clostridium acetobutylicum ATCC 824. Non-replicative plasmid constructs, either clostridial phosphotransacetylase (pta) or butyrate kinase (buk) gene fragments, were integrated into homologous regions on the chromosome. Integration was assumed to occur by a Campbell-like mechanism, inactivating either pta or buk. Inactivation of the pta gene reduced phosphotransacetylase and acetate kinase activity and significantly decreased acetateproduction. Inactivation of the buk gene reduced butyrate kinase activity, significantly decreased butyrate production and increased butanol production.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 27/05/20 alle ore 02:01:43