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Titolo:
EFFECTS OF SEQUENCE CONTEXTS ON MISINCORPORATION OF NUCLEOTIDES OPPOSITE 2-HYDROXYADENINE
Autore:
KAMIYA H; KASAI H;
Indirizzi:
UNIV OCCUPAT & ENVIRONM HLTH,DEPT ENVIRONM ONCOL,INST IND ECOL SCI,YAHATANISHI KU,1-1 ISEIGAOKA KITAKYUSHU FUKUOKA 807 JAPAN UNIV OCCUPAT & ENVIRONM HLTH,DEPT ENVIRONM ONCOL,INST IND ECOL SCI,YAHATANISHI KU KITAKYUSHU FUKUOKA 807 JAPAN
Titolo Testata:
FEBS letters
fascicolo: 1-2, volume: 391, anno: 1996,
pagine: 113 - 116
SICI:
0014-5793(1996)391:1-2<113:EOSCOM>2.0.ZU;2-Q
Fonte:
ISI
Lingua:
ENG
Soggetto:
C-HA-RAS; ESCHERICHIA-COLI; 8-HYDROXYGUANINE 7,8-DIHYDRO-8-OXOGUANINE; OXYGEN RADICALS; DNA-SYNTHESIS; HOT-SPOTS; LACI GENE; MUTATIONS; KINETICS; CELLS;
Keywords:
2-HYDROXYADENINE; OXIDATIVE DNA DAMAGE; MISINCORPORATION; DNA POLYMERASE; SEQUENCE CONTEXT;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Science Citation Index Expanded
Citazioni:
21
Recensione:
Indirizzi per estratti:
Citazione:
H. Kamiya e H. Kasai, "EFFECTS OF SEQUENCE CONTEXTS ON MISINCORPORATION OF NUCLEOTIDES OPPOSITE 2-HYDROXYADENINE", FEBS letters, 391(1-2), 1996, pp. 113-116

Abstract

Twelve oligonucleotides containing 2-hydroxyadenine (2-OH-Ade) with different neighboring bases were used as templates in DNA polymerase reactions, and the effects of the sequence contexts were investigated. DNA polymerases alpha and beta inserted dTMP and dCMP opposite 2-OH-Adein most of the oligonucleotides tested. The Klenow fragment of DNA polymerase I primarily incorporated dTMP and dTMP, Effects of the 5'-flanking base of 2-OH-Ade was found when the 3'-flanking base of 2-OH-Adewas A or C, Incorporation of dAMP occurred when the oxidized base waslocated in a 5' -TAXA- 3' (A represents 2-OH-Ade) sequence. These results suggest that the formation of 2-OH-Ade in DNA may induce all themutations involving A (A --> G transition, and A --> T and A --> C transversions) in cells.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 03/07/20 alle ore 14:38:58