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Titolo:
INTERACTION STUDIES BETWEEN THE P21CIP1 WAF1 CYCLIN-DEPENDENT KINASE INHIBITOR AND PROLIFERATING CELL NUCLEAR ANTIGEN (PCNA) BY SURFACE-PLASMON RESONANCE/
Autore:
KNIBIEHLER M; GOUBIN F; ESCALAS N; JONSSON ZO; MAZARGUIL H; HUBSCHER U; DUCOMMUN B;
Indirizzi:
UNIV TOULOUSE 3,INST PHARMACOL & BIOL STRUCT,205 ROUTE NARBONNE F-31077 TOULOUSE FRANCE UNIV TOULOUSE 3,INST PHARMACOL & BIOL STRUCT F-31077 TOULOUSE FRANCE UNIV ZURICH IRCHEL,INST VET BIOCHEM CH-8057 ZURICH SWITZERLAND
Titolo Testata:
FEBS letters
fascicolo: 1-2, volume: 391, anno: 1996,
pagine: 66 - 70
SICI:
0014-5793(1996)391:1-2<66:ISBTPW>2.0.ZU;2-B
Fonte:
ISI
Lingua:
ENG
Soggetto:
DNA-SYNTHESIS; BINDING; SYSTEM; IDENTIFICATION; PROTEIN; DOMAINS;
Keywords:
PCNA; CYCLIN-DEPENDENT KINASE INHIBITOR; P21CIP1/WAF1; CELL CYCLE;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Science Citation Index Expanded
Citazioni:
19
Recensione:
Indirizzi per estratti:
Citazione:
M. Knibiehler et al., "INTERACTION STUDIES BETWEEN THE P21CIP1 WAF1 CYCLIN-DEPENDENT KINASE INHIBITOR AND PROLIFERATING CELL NUCLEAR ANTIGEN (PCNA) BY SURFACE-PLASMON RESONANCE/", FEBS letters, 391(1-2), 1996, pp. 66-70

Abstract

The cyclin-dependent kinase (CDK) inhibitor p21Cip1 consists of two domains that interact with CDKs and proliferating cell nuclear antigen (PCNA), respectively, We have investigated the interaction between p21Cip1 and PCNA using surface plasmon resonance (SPR) technology and compared the results with those obtained from other sources such as the yeast two-hybrid system, Whilst other methods are only semi-quantitative, the SPR technique allowed us to determine the kinetic parameters ofthe interaction, The apparent equilibrium constant K-D calculated forthese kinetic parameters was 3.2 x 10(-7) M, We further demonstrate the use of SPR to study the interaction between mutant proteins and to determine their actual K-D, The interaction between p21Cip1/PCNA is shown to be dependent upon the trimeric conformation of PCNA since a point mutant that abolishes PCNA-PCNA interaction also abolishes PCNA's interaction with p21Cip1, Finally, we demonstrate that SPR can be used to characterise the interaction of p21Cip1 and PCNA in the presence ofshort competitive peptides.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 26/09/20 alle ore 01:15:56