Catalogo Articoli (Spogli Riviste)

OPAC HELP

Titolo:
LOCALIZATION OF THE MESSENGER-RNA ENCODING THE ZONA PROTEIN ZP3-ALPHAIN THE PORCINE OVARY, OOCYTE AND EMBRYO BY NONRADIOACTIVE IN-SITU HYBRIDIZATION
Autore:
KOLLE S; SINOWATZ F; BOIE G; TOTZAUER I; AMSELGRUBER W; PLENDL J;
Indirizzi:
UNIV MUNICH,DEPT VET ANAT,VET STR 13 D-80539 MUNICH GERMANY
Titolo Testata:
Histochemical Journal
fascicolo: 6, volume: 28, anno: 1996,
pagine: 441 - 447
SICI:
0018-2214(1996)28:6<441:LOTMET>2.0.ZU;2-E
Fonte:
ISI
Lingua:
ENG
Soggetto:
ACROSOME REACTION; MACROMOLECULAR-COMPOSITION; PIG OOCYTES; PELLUCIDA PROTEINS; ALPHA-GLYCOPROTEIN; SPERM RECEPTOR; ABSOLUTE RATES; MOUSE OOCYTES; IMMUNIZATION; INHIBITION;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Citazioni:
49
Recensione:
Indirizzi per estratti:
Citazione:
S. Kolle et al., "LOCALIZATION OF THE MESSENGER-RNA ENCODING THE ZONA PROTEIN ZP3-ALPHAIN THE PORCINE OVARY, OOCYTE AND EMBRYO BY NONRADIOACTIVE IN-SITU HYBRIDIZATION", Histochemical Journal, 28(6), 1996, pp. 441-447

Abstract

The mRNA of the zona pellucida glycoprotein ZP3 alpha was localized in frozen sections of pig ovaries, isolated oocytes and early embryos by in situ hybridization using biotinylated oligonucleotide probes. In follicles, the distribution of mRNA for ZP3 alpha was correlated with the developmental stage: in primordial and primary follicles, the mRNAwas shown to be predominantly localized in the oocyte. In secondary follicles, mRNA was found in both the oocyte and follicle cells. In tertiary and preovulatory follicles, the follicle cells showed distinct staining, whereas the oocyte was labelled weakly. In the early embryo, i.e. 2 days after fertilization, mRNA for ZP3 alpha could not be demonstrated. Our results suggest that, in the pig, the zona pellucida protein ZP3 alpha is synthesized by the oocyte and the follicle cells in sequence. After fertilization, synthesis of ZP3 alpha is terminated.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 05/12/20 alle ore 16:17:18