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Titolo:
QUANTITATIVE METHOD FOR ENUMERATION OF BR EVIBACTERIUM LINENS CELLS IN BACTERIAL SURFACE-RIPENED CHEESE
Autore:
OUMER A; LECLERCQPERLAT MN; BERGERE JL; CORRIEU G;
Indirizzi:
INRA,LAB GENIE & MICROBIOL PROCEDES ALIMENTAIRES F-78850 THIVERVAL GRIGNON FRANCE
Titolo Testata:
Le Lait
fascicolo: 4, volume: 76, anno: 1996,
pagine: 389 - 404
SICI:
0023-7302(1996)76:4<389:QMFEOB>2.0.ZU;2-X
Fonte:
ISI
Lingua:
FRE
Soggetto:
MICROORGANISMS; MILK; PH;
Keywords:
BREVIBACTERIUM LINENS; CELLULAR CONCENTRATION; CELL PIGMENT QUANTITY; EXTRACTION BY METHANOL; BACTERIAL SURFACE-RIPENED CHEESE;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Citazioni:
28
Recensione:
Indirizzi per estratti:
Citazione:
A. Oumer et al., "QUANTITATIVE METHOD FOR ENUMERATION OF BR EVIBACTERIUM LINENS CELLS IN BACTERIAL SURFACE-RIPENED CHEESE", Le Lait, 76(4), 1996, pp. 389-404

Abstract

A method for enumeration of B linens cells ATCC 9175 in surface of bacterial surface-ripened cheese by estimation of pigment quantity was developed. In first time, this method was realized in liquid medium, after that applied in cheese. The cellular pigments of B linens were completely solubilized by methanol. The best conditions of solubility were 1 h at 25 degrees C from cell liquid culture. in cheese, it is necessary to eliminate the fat content by the methanol solution by standingit I h at 4 degrees C before filtration. The cellular pigmentation was measured by absorbance at 450 nm. Absorbance measure at 450 nm was equivalent to level of pigmentation. The pigmentation of B linens ATCC 9175 was independent of light, of sodium chloride concentration and growth medium (liquid or cheese). In liquid medium with similar composition of curd, the cell dry weight matter and turbidimetric absorbance at 650 nm were chosen as reference methods. The dry weight method couldnot be used because the correlation established between pigment quantity and dry weight were different for each test. The correlation established between turbidimetric absorbance at 650 nm and pigment quantitywas satisfying (R(2) greater than or equal to 0.90) and independent of the test considered. The pigment quantity method by absorbance at 450 nm could be used for rapid determination of total cell concentrationof B linens ATCC 9175 in experimental bacterial surface-ripened cheeses during ripening. This method would be applicable to stable pigmentation B linens strains. In the case of microbial mixtures like that existing in the surface of industrial cheese, this pigment quantity method could be used only if the other bacteria did not produce pigments.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 06/07/20 alle ore 04:40:12