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Titolo:
DIRECT VISUALIZATION OF COPPER-METALLOTHIONEIN IN LEC RAT KIDNEYS - APPLICATION OF AUTOFLUORESCENCE SIGNAL OF COPPER-THIOLATE CLUSTER
Autore:
OKABE M; NAKAYAMA K; KURASAKI M; YAMASAKI F; AOYAGI K; YAMANOSHITA O; SATO S; OKUI T; OHYAMA T; KASAI N;
Indirizzi:
HOKKAIDO UNIV,GRAD SCH ENVIRONM EARTH SCI,DEPT ENVIRONM MED & INFORMAT,KITA KU,KITA 10,NISHI 5 SAPPORO HOKKAIDO 060 JAPAN HOKKAIDO INST PUBL HLTH,DIV LIFE SCI SAPPORO HOKKAIDO JAPAN HOKKAIDO INST PUBL HLTH,DIV BIOTECHNOL SAPPORO HOKKAIDO JAPAN TOHOKU UNIV,INST ANIM EXPERIMENTAT SENDAI MIYAGI JAPAN
Titolo Testata:
The Journal of histochemistry and cytochemistry
fascicolo: 8, volume: 44, anno: 1996,
pagine: 865 - 873
SICI:
0022-1554(1996)44:8<865:DVOCIL>2.0.ZU;2-6
Fonte:
ISI
Lingua:
ENG
Soggetto:
EVANS CINNAMON RATS; CADMIUM-METALLOTHIONEIN; TIME COURSE; LIVER; HEPATITIS; ACCUMULATION; SPECTROSCOPY; METABOLISM; INDUCTION; TOXICITY;
Keywords:
METALLOTHIONEIN; COPPER; AUTOFLUORESCENCE; LEC RAT; KIDNEY; IN SITU BLOTTING; MESSENGER-RNA DISTRIBUTION;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Citazioni:
45
Recensione:
Indirizzi per estratti:
Citazione:
M. Okabe et al., "DIRECT VISUALIZATION OF COPPER-METALLOTHIONEIN IN LEC RAT KIDNEYS - APPLICATION OF AUTOFLUORESCENCE SIGNAL OF COPPER-THIOLATE CLUSTER", The Journal of histochemistry and cytochemistry, 44(8), 1996, pp. 865-873

Abstract

We report on the histochemistry of copper-metallothionein (Cu-MT) in the kidneys of Long Evans Cinnamon (LEG) rats, We used the visualization principle of histochemistry based on the autofluorescence emission from the fluorophore of Cu+-thiolate dusters in proteins, intense autofluorescence signals were observed with a ring at the outer stripe of the outer medulla. Orange fluorescence signals were observed in the nuclei and cytoplasm of proximal straight tubular (PST) cells of segment3 (S3) at the outer stripe of the outer medulla, and yellow-orange signals were detected in lysosome-like organelles in the proximal convoluted tubule (PCT) cells of segments 1 and 2 (S1 and S2) adjacent to the glomeruli in the cortex, These fluorescent materials were identifiedas Cu-MT because both signals were quenched by withdrawing Cu+ or by blocking cysteine residues, the distributions of cysteine residues andimmunoreactive MT showed identical patterns to the localization of the fluorescence signals, and the fluorescent proteins containing Cu were eluted at the same Kd value of purified Cu-MT by gel filtration chromatography, However, a high level of MT mRNA was detected only in the outer stripe of the outer medulla where the orange fluorescence signals were detected, but not in the cortex, This difference in localization between the protein and the mRNA suggested that synthesis of renal MT occurs do novo in the outer stripe of the outer medulla, The yellow-orange fluorescent Cu-MT located in the lysosomal organelles at St andS2 of the PCT cells in the cortex could be Cu-MT of nonrenal origin, i.e., Cu-MT transported from other organs.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 02/12/20 alle ore 14:20:24