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Titolo:
QUANTITATION OF L-SELECTIN DISTRIBUTION ON HUMAN-LEUKOCYTE MICROVILLIBY IMMUNOGOLD LABELING AND ELECTRON-MICROSCOPY
Autore:
BRUEHL RE; SPRINGER TA; BAINTON DF;
Indirizzi:
UNIV CALIF SAN FRANCISCO,DEPT PATHOL,BOX 0506 SAN FRANCISCO CA 94143 HARVARD UNIV,SCH MED,CTR BLOOD RES INC BOSTON MA 00000 HARVARD UNIV,SCH MED,DEPT PATHOL BOSTON MA 00000
Titolo Testata:
The Journal of histochemistry and cytochemistry
fascicolo: 8, volume: 44, anno: 1996,
pagine: 835 - 844
SICI:
0022-1554(1996)44:8<835:QOLDOH>2.0.ZU;2-T
Fonte:
ISI
Lingua:
ENG
Soggetto:
LYMPHOCYTE HOMING RECEPTOR; HIGH ENDOTHELIAL VENULES; NEUTROPHIL ADHESION; SPATIAL-DISTRIBUTION; CHEMOTACTIC FACTORS; PHYSIOLOGICAL FLOW; CYTOPLASMIC DOMAIN; CELLS; EXPRESSION; LECAM-1;
Keywords:
HUMAN; L-SELECTIN; NEUTROPHIL; MONOCYTE; LYMPHOCYTE; BASOPHIL; EOSINOPHIL; MICROVILLI; CELL ADHESION; IMMUNOCYTOCHEMISTRY;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Citazioni:
48
Recensione:
Indirizzi per estratti:
Citazione:
R.E. Bruehl et al., "QUANTITATION OF L-SELECTIN DISTRIBUTION ON HUMAN-LEUKOCYTE MICROVILLIBY IMMUNOGOLD LABELING AND ELECTRON-MICROSCOPY", The Journal of histochemistry and cytochemistry, 44(8), 1996, pp. 835-844

Abstract

L-Selectin is a leukocyte cell adhesion receptor that contributes to neutrophil (PMN) rolling on activated endothelium at sites of inflammation and mediates lymphocyte attachment to high endothelial venules inperipheral lymph nodes, Localization of this receptor to the tips of PMN and lymphocyte microvilli has been demonstrated. However, its distribution on these cells has not been quantified, and its localization on other leukocytes and the morphometry of microvilli on different leukocyte subpopulations have not been previously examined. In this study, PMN and mononuclear leukocytes were isolated from anticoagulated blood by dextran sedimentation and density centrifugation, fixed in 2% paraformaldehyde and 0.05% glutaraldehyde, immunogold-labeled for L-selectin, and embedded in Epon resin. The distribution of L-selectin was determined by counting gold I;articles on the plasma membrane of sectioned cells, and the surface microstructure of these cells was surveyed on two-dimensional transmission electron micrographs, On average, 78% of PMN, 72% of monocyte, and 71% of lymphocyte L-selectin was observedon the microvilli, with more variance on lymphocytes than the other cell types. Typical PMN and monocyte sections had 26 microvilli, whereas typical lymphocyte sections had 23. Quantitation of the distributionof L-selectin and leukocyte surface topology offers a foundation fromwhich to study the requirement of microvilli or microvillus-localizedL-selectin for leukocyte tethering and rolling in model systems that mimic microvascular environments.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 20/01/21 alle ore 03:08:36