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Titolo:
FIBRILLIN-1 AND FIBULIN-2 INTERACT AND ARE COLOCALIZED IN SOME TISSUES
Autore:
REINHARDT DP; SASAKI T; DZAMBA BJ; KEENE DR; CHU ML; GOHRING W; TIMPL R; SAKAI LY;
Indirizzi:
SHRINERS HOSP CRIPPLED CHILDREN PORTLAND OR 97201 SHRINERS HOSP CRIPPLED CHILDREN PORTLAND OR 97201 MAX PLANCK INST BIOCHEM D-82152 MARTINSRIED GERMANY THOMAS JEFFERSON UNIV,DEPT BIOCHEM & MOL BIOL PHILADELPHIA PA 19107 THOMAS JEFFERSON UNIV,DEPT DERMATOL & CUTANEOUS BIOL PHILADELPHIA PA 19107 OREGON HLTH SCI UNIV,DEPT BIOCHEM & MOL BIOL PORTLAND OR 97201
Titolo Testata:
The Journal of biological chemistry
fascicolo: 32, volume: 271, anno: 1996,
pagine: 19489 - 19496
SICI:
0021-9258(1996)271:32<19489:FAFIAA>2.0.ZU;2-1
Fonte:
ISI
Lingua:
ENG
Soggetto:
EXTRACELLULAR-MATRIX PROTEIN; BETA-BINDING-PROTEIN; EGF-LIKE REPEATS; MARFAN-SYNDROME; ELASTIC FIBERS; STRUCTURAL CHARACTERIZATION; NIDOGEN AFFINITY; CALCIUM-BINDING; GENE-PRODUCT; MICROFIBRILS;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Citazioni:
46
Recensione:
Indirizzi per estratti:
Citazione:
D.P. Reinhardt et al., "FIBRILLIN-1 AND FIBULIN-2 INTERACT AND ARE COLOCALIZED IN SOME TISSUES", The Journal of biological chemistry, 271(32), 1996, pp. 19489-19496

Abstract

Microfibrils 10-12 nm in diameter are found in elastic and non-elastic tissues with fibrillin as a major component. Little is known about the supramolecular structure of these microfibrils and the protein interactions it is based on. To identify protein binding ligands of fibrillin-1, we tested binding of recombinant fibrillin-1 peptides to different extracellular matrix proteins in solid phase assays. Among the proteins tested, only fibulin-2 showed significant binding to rF11, the N-terminal half of fibrillin-1, in a calcium-dependent manner. Surface plasmon resonance demonstrated high affinity binding with a K-d=56 nM. With overlapping recombinant fibrillin-1 peptides, the binding site for fibulin-2 was narrowed down to the N terminus of fibrillin-1 (aminoacid positions 45-450). Immunofluorescence in tissues demonstrated colocalization of fibrillin and fibulin-2 in skin, perichondrium, elastic intima of blood vessels, and kidney glomerulus. Fibulin-2 was not present in ocular ciliary zonules, tendon, and the connective tissue around kidney tubules and lung alveoli, which all contain fibrillin. Immunogold labeling of fibulin-2 on microfibrils in skin was found preferentially at the interface between microfibrils and the amorphous elastin core, suggesting that in vivo the interaction between fibrillin-1 and fibulin-2 is regulated by cellular expression and deposition as wellas by protein-protein interactions.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 25/11/20 alle ore 07:32:36