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Titolo:
MANNITOL, A NOVEL BACTERIAL COMPATIBLE SOLUTE IN PSEUDOMONAS-PUTIDA S12
Autore:
KETS EPW; GALINSKI EA; DEWIT M; DEBONT JAM; HEIPIEPER HJ;
Indirizzi:
DLO,ATO,AGROTECHNOL RES INST,BORNSESTEEG 59,POB 17 NL-6700 AA WAGENINGEN NETHERLANDS AGR UNIV WAGENINGEN,DEPT FOOD SCI,DIV IND MICROBIOL NL-6700 EV WAGENINGEN NETHERLANDS UNIV BONN,INST MIKROBIOL & BIOTECHNOL D-5300 BONN GERMANY
Titolo Testata:
Journal of bacteriology
fascicolo: 23, volume: 178, anno: 1996,
pagine: 6665 - 6670
SICI:
0021-9193(1996)178:23<6665:MANBCS>2.0.ZU;2-O
Fonte:
ISI
Lingua:
ENG
Soggetto:
HALOPHILIC EUBACTERIA; STRESS PROTECTION; OSMOTIC-STRESS; FATTY-ACIDS; OSMOLYTES; TREHALOSE; CELLS; TETRAHYDROPYRIMIDINES; OSMOREGULATION; STABILIZATION;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Citazioni:
36
Recensione:
Indirizzi per estratti:
Citazione:
E.P.W. Kets et al., "MANNITOL, A NOVEL BACTERIAL COMPATIBLE SOLUTE IN PSEUDOMONAS-PUTIDA S12", Journal of bacteriology, 178(23), 1996, pp. 6665-6670

Abstract

The aim of this study was to identify the compatible solutes accumulated by Pseudomonas putida S12 subjected to osmotic stress, In responseto reduced water activity, P. putida S12 accumulated N alpha-acetylglutaminylglutamine amide (NAGGN) simultaneously with a novel compatiblesolute identified as mannitol (using C-13- and H-1-uuclear magnetic resonance, liquid chromatography-mass spectroscopy and high-performanceliquid chromatography methods) to maximum concentrations of 74 and 258 mu mol g (dry weight) of cells(-1), respectively, The intracellular amounts of each solute varied with both the type and amount of osmolyte applied to induce osmotic stress in the medium, Both solutes were synthesized de novo, Addition of betaine to the medium resulted in accumulation of this compound and depletion of both NAGGN and mannitol, Mannitol and NAGGN were accumulated when sucrose instead of salts was used to reduce the medium water activity, Furthermore, both compatible solutes were accumulated when glucose was substituted by other carbon sources, However, the intracellular quantities of mannitol decreased when fructose, succinate, or lactate were applied as a carbon source, Mannitol,vas also raised to high intracellular concentrations by other salt-stressed Pseudomonas putida strains, This is the first study demonstrating a principal role for the de novo-synthesized polyol mannitol in osmoadaptation of a heterotrophic eubacterium.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 29/11/20 alle ore 03:34:24