Catalogo Articoli (Spogli Riviste)

OPAC HELP

Titolo:
FURTHER-STUDIES ON THE MECHANISM OF ACTION OF THE ENDOGENOUS BENZODIAZEPINE RECEPTOR-LIGAND OCTADECANEUROPEPTIDE ON GONADOTROPIN-RELEASING-HORMONE GENE-EXPRESSION IN THE RAT-BRAIN
Autore:
LI SY; PELLETIER G;
Indirizzi:
CHU LAVAL,RES CTR,MRC,GRP MOL ENDOCRINOL,2705 LAURIER BLVD QUEBEC CITY PQ G1V 4G2 CANADA CHU LAVAL,RES CTR,MRC,GRP MOL ENDOCRINOL QUEBEC CITY PQ G1V 4G2 CANADA UNIV LAVAL QUEBEC CITY PQ G1K 7P4 CANADA
Titolo Testata:
Neuroendocrinology
fascicolo: 2, volume: 64, anno: 1996,
pagine: 79 - 84
SICI:
0028-3835(1996)64:2<79:FOTMOA>2.0.ZU;2-2
Fonte:
ISI
Lingua:
ENG
Soggetto:
BINDING INHIBITOR DBI; INSITU HYBRIDIZATION; MESSENGER-RNA; DIAZEPAM; LOCALIZATION; ODN; INVOLVEMENT; PITUITARY; PEPTIDES; SITES;
Keywords:
BENZODIAZEPINES; BENZODIAZEPINE RECEPTORS; GONADOTROPIN-RELEASING HORMONE MESSENGER-RNA; GAMMA-AMINOBUTYRIC ACID; FLUMAZENIL; GONADOTROPIN-RELEASING HORMONE; MOLECULAR NEUROENDOCRINOLOGY;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Science Citation Index Expanded
Citazioni:
25
Recensione:
Indirizzi per estratti:
Citazione:
S.Y. Li e G. Pelletier, "FURTHER-STUDIES ON THE MECHANISM OF ACTION OF THE ENDOGENOUS BENZODIAZEPINE RECEPTOR-LIGAND OCTADECANEUROPEPTIDE ON GONADOTROPIN-RELEASING-HORMONE GENE-EXPRESSION IN THE RAT-BRAIN", Neuroendocrinology, 64(2), 1996, pp. 79-84

Abstract

We have recently reported that the intracerebroventricular injection of the endogenous benzodiazepine (BZD) receptor ligand octadecaneuropeptide (ODN) (30 mu g/kg b.w.) could induce a decrease in gonadotropin-releasing hormone (GnRH) mRNA levels in the male rat brain. This inhibitory effect could be completely reversed by the concomitant administration of the GABA(A) antagonist picrotoxin. In order to further investigate the mechanism of action of ODN, we investigated the effects of intracerebroventricular injection of a smaller dose of ODN (3 mu g/kg b.w.) and the influence of the GABA(A) receptor agonist muscimol and the antagonist to BZD receptors flumazenil on GnRH gene expression. Treatment with ODN induced a 40% decrease in mRNA levels, an effect which was potentiated by the concomitant administration of muscimol, The administration of flumazenil produced a small increase in GnRH mRNA whilethe inhibitory effect of ODN on GnRH mRNA levels was completely prevented by the administration of this BZD antagonist. Moreover, the intravenous administration of increasing doses of ODN also induced a markeddecrease of 33, 32 and 38% for 75, 150 and 300 mu g/kg b.w., an effect which was completely abolished by picrotoxin. These data clearly indicate that an endogenous neuropeptide can activate the GABA(A) BZD receptor complex to negatively modulate the activity of GnRH neurons. They also suggest that this peptide or other endogenous activators of theGABA(A) BZD receptors might exert a tonic inhibitory influence on GnRH gene expression.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 25/11/20 alle ore 04:18:48