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Titolo:
REFOLDING PARAMETERS FOR THE ALLOSTERIC HOMODIMERIC GUANYLYL CYCLASE CATALYTIC CORE FROM THE ATRIAL-NATRIURETIC-PEPTIDE RECEPTOR
Autore:
THORPE DS; NIU S; MORKIN E;
Indirizzi:
SELECTIDE CORP,DEPT DISCOVERY BIOL,1580 E HANLEY BLVD TUCSON AZ 85737 SELECTIDE CORP,DEPT CHEM TUCSON AZ 85737 UNIV ARIZONA,COLL MED,ARIZONA HLTH SCI CTR,DEPT PHYSIOL TUCSON AZ 85724 UNIV ARIZONA,COLL MED,ARIZONA HLTH SCI CTR,DEPT INTERNAL MED TUCSON AZ 85724 UNIV ARIZONA,COLL MED,ARIZONA HLTH SCI CTR,UNIV HEART CTR TUCSON AZ 85724
Titolo Testata:
Biochemical and biophysical research communications
fascicolo: 3, volume: 224, anno: 1996,
pagine: 765 - 771
SICI:
0006-291X(1996)224:3<765:RPFTAH>2.0.ZU;2-L
Fonte:
ISI
Lingua:
ENG
Soggetto:
ESCHERICHIA-COLI; PROTEINS; DOMAIN;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Science Citation Index Expanded
Citazioni:
18
Recensione:
Indirizzi per estratti:
Citazione:
D.S. Thorpe et al., "REFOLDING PARAMETERS FOR THE ALLOSTERIC HOMODIMERIC GUANYLYL CYCLASE CATALYTIC CORE FROM THE ATRIAL-NATRIURETIC-PEPTIDE RECEPTOR", Biochemical and biophysical research communications, 224(3), 1996, pp. 765-771

Abstract

Protein folding continues to be an important biophysical topic in molecular biology. We report the parameters for successfully refolding the guanylyl cyclase core of the ANP receptor, an allosteric homodimericenzyme. Urea was a better chaotropic solvent than guanidine HCl, and physiological salt concentrations and pH were needed for optimal recovery of enzymatic activity. Renaturation was more sensitive to alkalinecompared to acidic deviations in solvent conditions. The time course of refolding was sigmoidal producing an enzyme with a specific activity of 16,000 pmol cGMP/min/mg using 60 mu M concentration of substrate. Additional factors are described in this unusual case of renaturing an allosteric homodimeric enzyme in vitro. (C) 1996 Academic Press, Inc.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 24/09/20 alle ore 01:32:39