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Titolo:
CHANGES IN LECTIN-BINDING TO BOVINE SPERM DURING HEPARIN-INDUCED CAPACITATION
Autore:
MEDEIROS CMO; PARRISH JJ;
Indirizzi:
UNIV WISCONSIN,DEPT MEAT & ANIM SCI MADISON WI 53706 UNIV WISCONSIN,DEPT MEAT & ANIM SCI MADISON WI 53706
Titolo Testata:
Molecular reproduction and development
fascicolo: 4, volume: 44, anno: 1996,
pagine: 525 - 532
SICI:
1040-452X(1996)44:4<525:CILTBS>2.0.ZU;2-E
Fonte:
ISI
Lingua:
ENG
Soggetto:
INVITRO CAPACITATION; ACROSOME REACTION; INTRACELLULAR PH; SPERMATOZOA; SURFACE; PROTEINS; HAMSTER; OVIDUCT; FLUID;
Keywords:
WHEAT GERM AGGLUTININ; PROTAMINE; GLUCOSE; PROPIDIUM IODIDE;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Science Citation Index Expanded
Science Citation Index Expanded
Science Citation Index Expanded
Citazioni:
28
Recensione:
Indirizzi per estratti:
Citazione:
C.M.O. Medeiros e J.J. Parrish, "CHANGES IN LECTIN-BINDING TO BOVINE SPERM DURING HEPARIN-INDUCED CAPACITATION", Molecular reproduction and development, 44(4), 1996, pp. 525-532

Abstract

Changes in the plasma membrane of bovine sperm during heparin-inducedcapcitation were detected by the binding of fluorescent labeled lectins to unfixed sperm. Of the seven lectins evaluated, only binding of wheat germ agglutinin (WGA) changed with capacitation. Sperm were classified into one of 5 patterns (p1-p5) based on staining with WGA, presence or absence of propidium iodide (PI) staining (dead or alive), and acrosomal integrity (acrosome intact or reacted). The major changes associated with capacitation occurred in p1 and p2. Sperm in p1 exhibited diffuse WGA binding over the anterior sperm head, were alive, and had intact acrosomes. In p2, sperm were also acrosome intact and alive, but lacked WGA binding. When sperm were incubated under capacitating conditions with heparin, there was a decrease over time in the percentage of sperm classified in p1 (p < 0.05) and an increase in the percentage of sperm in p2 (p < 0.05). When capacitation by heparin was delayed by the inclusion of glucose in the culture medium, the same heparin-dependent changes in the percentage of sperm in p1 and p2 were delayed(p < 0.05). When capacitation by heparin was inhibited by including protamine in the culture medium, the percentage of sperm in p1 or p2 was not different from sperm incubated without heparin. Heparin-induced capacitation was associated with a loss of WGA binding to the bovine sperm head. (C) 1996 Wiley-Liss, Inc.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 29/11/20 alle ore 01:59:18