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Titolo:
MAMMALIAN SUG1 AND C-FOS IN THE NUCLEAR 26S PROTEASOME
Autore:
WANG WL; CHEVRAY PM; NATHANS D;
Indirizzi:
JOHNS HOPKINS UNIV,SCH MED,HOWARD HUGHES MED INST,725 N WOLFE ST BALTIMORE MD 21205 JOHNS HOPKINS UNIV,SCH MED,HOWARD HUGHES MED INST BALTIMORE MD 21205 JOHNS HOPKINS UNIV,SCH MED,DEPT MOL BIOL & GENET BALTIMORE MD 21205
Titolo Testata:
Proceedings of the National Academy of Sciences of the United Statesof America
fascicolo: 16, volume: 93, anno: 1996,
pagine: 8236 - 8240
SICI:
0027-8424(1996)93:16<8236:MSACIT>2.0.ZU;2-7
Fonte:
ISI
Lingua:
ENG
Soggetto:
TAT-BINDING-PROTEIN; OSTEO-SARCOMA VIRUS; RNA-POLYMERASE-II; IN-VIVO; MULTICATALYTIC PROTEINASE; MEDIATED TRANSACTIVATION; CONJUGATING ENZYMES; MOLECULAR-CLONING; 26-S PROTEASE; YEAST;
Keywords:
TRANSCRIPTION; LEUCINE ZIPPER;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Citazioni:
39
Recensione:
Indirizzi per estratti:
Citazione:
W.L. Wang et al., "MAMMALIAN SUG1 AND C-FOS IN THE NUCLEAR 26S PROTEASOME", Proceedings of the National Academy of Sciences of the United Statesof America, 93(16), 1996, pp. 8236-8240

Abstract

In a search for regulatory proteins that interact with the leucine zipper motif of c-Fos in the yeast two-hybrid screen, ive have identified a protein (FZA-B) that has extensive sequence similarity to SUG1 of Saccharomyces cerevisiae. Here we show that FZA-B can functionally substitute for SUG1 in yeast and that FZA-B interacts with Fos proteins in vitro through their leucine zippers. in rat liver and in HeLa cells,FZA-B is present in the 26S proteasome complex, as is c-Fos. Immobilized antibody raised against an FZA-B-specific peptide depleted peptidase activity, proteasomal proteins, FZA-B, and c-Fos from a 26S proteasome preparation. FZA-B is found predominantly in the nuclear fraction of COS cells expressing an FZA-B transgene and in the nuclear 26S proteasome of HeLa cells. We conclude that FZA-B is the mammalian homolog of SUG1 (mSug1) and that it is present in the nuclear 26S proteasome of cells. Our results suggest that mSug1 may be involved in the degradation of c-Fos and other transcription factors.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 20/09/20 alle ore 07:10:56