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Titolo:
ENVOPLAKIN, A NOVEL PRECURSOR OF THE CORNIFIED ENVELOPE THAT HAS HOMOLOGY TO DESMOPLAKIN
Autore:
RUHRBERG C; HAJIBAGHERI MAN; SIMON M; DOOLEY TP; WATT FM;
Indirizzi:
IMPERIAL CANC RES FUND,KERATINOCYTE LAB,44 LINCOLNS INN FIELDS LONDONWC2A 3PX ENGLAND IMPERIAL CANC RES FUND,KERATINOCYTE LAB LONDON WC2A 3PX ENGLAND IMPERIAL CANC RES FUND,ELECTRON MICROSCOPY UNIT LONDON WC2A 3PX ENGLAND SUNY STONY BROOK,HLTH SCI CTR,SCH MED,DEPT DERMATOL,DEPT ORAL BIOL & PATHOL,SCH DENT MED STONY BROOK NY 11794 SO RES INST,DEPT MOL PHARMACOL BIRMINGHAM AL 35205
Titolo Testata:
The Journal of cell biology
fascicolo: 3, volume: 134, anno: 1996,
pagine: 715 - 729
SICI:
0021-9525(1996)134:3<715:EANPOT>2.0.ZU;2-M
Fonte:
ISI
Lingua:
ENG
Soggetto:
BULLOUS PEMPHIGOID ANTIGEN; KERATIN INTERMEDIATE FILAMENTS; KIDNEY EPITHELIAL-CELLS; CROSS-LINKED ENVELOPE; HUMAN EPIDERMAL-CELLS; TERMINAL DIFFERENTIATION; SPATIAL REGULATION; DESMOSOMAL PLAQUE; PROTEIN-STRUCTURE; CULTURED-CELLS;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Citazioni:
58
Recensione:
Indirizzi per estratti:
Citazione:
C. Ruhrberg et al., "ENVOPLAKIN, A NOVEL PRECURSOR OF THE CORNIFIED ENVELOPE THAT HAS HOMOLOGY TO DESMOPLAKIN", The Journal of cell biology, 134(3), 1996, pp. 715-729

Abstract

The cornified envelope is a layer of transglutaminase cross-linked protein that is deposited under the plasma membrane of keratinocytes in the outermost layers of the epidermis. We present the sequence of one of the cornified envelope precursors, a protein with an apparent molecular mass of 210 kD. The 210-kD protein is translated from a 6.5-kb mRNA that is transcribed from a single copy gene. The mRNA was upregulated during suspension-induced terminal differentiation of cultured human keratinocytes. Like other envelope precursors, the 210-kD protein became insoluble in SDS and beta-mercaptoethanol on activation of transglutaminases in cultured keratinocytes. The protein was expressed in keratinizing and nonkeratinizing stratified squamous epithelia, but not in simple epithelia or nonepithelial cells, Immunofluorescence staining showed that in epidermal keratinocytes, both in vivo and in culture,the protein was upregulated during terminal differentiation and partially colocalized with desmosomal proteins. Immunogold EM confirmed thecolocalization of the 210-kD protein and desmoplakin at desmosomes and on keratin filaments throughout the differentiated layers of the epidermis, Sequence analysis showed that the 210-kD protein is homologousto the keratin-binding proteins desmoplakin, bullous pemphigoid antigen 1, and plectin. These data suggest that the 210-kD protein may linkthe cornified envelope to desmosomes and keratin filaments. We propose that the 210-kD protein be named ''envoplakin.''

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 09/08/20 alle ore 23:18:26