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Titolo:
IMMUNOGOLD LABELING OF NEUROENDOCRINE PEPTIDES WITH SPECIAL REFERENCETO ANTIBODY SPECIFICITY AND MULTIPLE STAINING TECHNIQUES
Autore:
LARSSON LI;
Indirizzi:
STATENS SERUM INST,DEPT MOL CELL BIOL,ARTILLERIVEY 5,BLDG 81 DK-2300 COPENHAGEN S DENMARK
Titolo Testata:
HISTOCHEMISTRY AND CELL BIOLOGY
fascicolo: 1, volume: 106, anno: 1996,
pagine: 93 - 103
SICI:
0948-6143(1996)106:1<93:ILONPW>2.0.ZU;2-C
Fonte:
ISI
Lingua:
ENG
Soggetto:
ELECTRON-MICROSCOPIC IMMUNOCYTOCHEMISTRY; 1.4-NM GOLD PARTICLES; HUMAN FETAL PANCREAS; CELL LUNG-CANCER; COLLOIDAL GOLD; ENDOCRINE-CELLS; LOW-TEMPERATURE; ULTRASTRUCTURAL-LOCALIZATION; ISLET HORMONES; SUBSTANCE-P;
Tipo documento:
Review
Natura:
Periodico
Citazioni:
73
Recensione:
Indirizzi per estratti:
Citazione:
L.I. Larsson, "IMMUNOGOLD LABELING OF NEUROENDOCRINE PEPTIDES WITH SPECIAL REFERENCETO ANTIBODY SPECIFICITY AND MULTIPLE STAINING TECHNIQUES", HISTOCHEM C, 106(1), 1996, pp. 93-103

Abstract

Immunogold methods have been very important for research on the neuroendocrine system. The compatibility of immunogold probes with optimal contrasting for electron microscopy has made localizations of neuroendocrine peptides to different subtypes of secretory organelles possibleand, currently, methods using covalent attachment of nanogold particles to antibodies and neuropeptide ligands hold promise for immunocytochemistry, receptor localizations and in situ hybridizations. Multihormonal phenotypes are a hallmark of both the developing and mature neuroendocrine system. The possibility to localize multiple coexisting messengers by multilabelling immunogold methods is emphasized, and different methods for achieving this are discussed. The most difficult part of immunocytochemistry involves definitions and interpretations of specificity, and a number of limitations and control procedures are discussed.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 10/07/20 alle ore 18:21:50