Catalogo Articoli (Spogli Riviste)

OPAC HELP

Titolo:
ROLES OF THE CONSERVED SERINES OF METALLOTHIONEIN IN CADMIUM-BINDING
Autore:
EMOTO T; KURASAKI M; OIKAWA S; SUZUKIKURASAKI M; OKABE M; YAMASAKI F; KOJIMA Y;
Indirizzi:
HOKKAIDO UNIV,GRAD SCH ENVIRONM EARTH SCI,DEPT ENVIRONM MED & INFORMAT,KITA KU,KITA-10,NISHI-5 SAPPORO HOKKAIDO 060 JAPAN HOKKAIDO UNIV,GRAD SCH ENVIRONM EARTH SCI,DEPT ENVIRONM MED & INFORMAT,KITA KU SAPPORO HOKKAIDO 060 JAPAN
Titolo Testata:
Biochemical genetics
fascicolo: 5-6, volume: 34, anno: 1996,
pagine: 239 - 251
SICI:
0006-2928(1996)34:5-6<239:ROTCSO>2.0.ZU;2-S
Fonte:
ISI
Lingua:
ENG
Soggetto:
ESCHERICHIA-COLI; MAMMALIAN METALLOTHIONEIN; DETOXIFICATION FUNCTION; METAL-BINDING; RAINBOW-TROUT; ALPHA-DOMAIN; EXPRESSION; LYSINES; GENE; REPLACEMENT;
Keywords:
ESCHERICHIA COLI; EXPRESSION; METAL-BINDING ABILITY; METALLOTHIONEIN; SERINE; MUTAGENESIS;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Science Citation Index Expanded
Citazioni:
30
Recensione:
Indirizzi per estratti:
Citazione:
T. Emoto et al., "ROLES OF THE CONSERVED SERINES OF METALLOTHIONEIN IN CADMIUM-BINDING", Biochemical genetics, 34(5-6), 1996, pp. 239-251

Abstract

The sequence of six amino acid residues -Ser-Cys-Cys-Ser-Cys-Cys- is present in all mammalian metallothionein sequences and has been highlyconserved during evolution, although the metallothioneins have divergent primary sequences. To determine whether two serines in the sequence play a crucial role in metal-binding of metallothioneins, a mutant metallothionein with these two serines replaced by leucines was obtained using an Escherichia coli expression system. The expressed protein was analyzed for its chemical and spectroscopic properties. It was confirmed that the mutant metallothionein (MT) bound cadmium through a metal-thiolate complex and that there was no strong difference between the mutant and the wild-type MTs in retaining the metal-binding cluster. However, the metal-binding cluster of the mutant metallothionein was more unstable than that of the wild-type metallothionein. The two conservative serines could play a role in the stability of metal-binding ligands.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 26/11/20 alle ore 14:13:03