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Titolo:
IDENTIFICATION OF LATENT MEMBRANE-PROTEIN 2A (LMP2A) DOMAINS ESSENTIAL FOR THE LMP2A DOMINANT-NEGATIVE EFFECT ON B-LYMPHOCYTE SURFACE-IMMUNOGLOBULIN SIGNAL-TRANSDUCTION
Autore:
FRUEHLING S; LEE SK; HERROLD R; FRECH B; LAUX G; KREMMER E; GRASSER FA; LONGNECKER R;
Indirizzi:
NORTHWESTERN UNIV,SCH MED,DEPT MICROBIOL IMMUNOL,303 E CHICAGO AVE CHICAGO IL 60611 NORTHWESTERN UNIV,SCH MED,DEPT MICROBIOL IMMUNOL CHICAGO IL 60611 UNIV SAARLAND,INST MED MIKROBIOL & HYG,ABT VIROL D-66421 HOMBURG GERMANY GSF FORSCHUNGSZENTRUM UNWELT & GESUNDHEIT,INST KLIN MOL BIOL & TUMORGENET D-81377 MUNICH GERMANY GSF MUNICH,INST IMMUNOL D-81377 MUNICH GERMANY
Titolo Testata:
Journal of virology
fascicolo: 9, volume: 70, anno: 1996,
pagine: 6216 - 6226
SICI:
0022-538X(1996)70:9<6216:IOLM2(>2.0.ZU;2-K
Fonte:
ISI
Lingua:
ENG
Soggetto:
EPSTEIN-BARR-VIRUS; GROWTH TRANSFORMATION INVITRO; RAT MONOCLONAL-ANTIBODIES; TYROSINE KINASES; MARMOSET LEUKOCYTES; ANTIGEN RECEPTOR; WW DOMAIN; GENE; CELLS; INFECTION;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Citazioni:
49
Recensione:
Indirizzi per estratti:
Citazione:
S. Fruehling et al., "IDENTIFICATION OF LATENT MEMBRANE-PROTEIN 2A (LMP2A) DOMAINS ESSENTIAL FOR THE LMP2A DOMINANT-NEGATIVE EFFECT ON B-LYMPHOCYTE SURFACE-IMMUNOGLOBULIN SIGNAL-TRANSDUCTION", Journal of virology, 70(9), 1996, pp. 6216-6226

Abstract

Epstein-Barr virus (EBV) recombinants which carry three different deletion mutations in the LMP2A cytoplasmic amino-terminal domain were constructed. The presence of each mutation, LMP2A Delta 21-36, LMP2A Delta 21-64, and LMP2A Delta 21-85, in EBV-infected transformed lymphoblastoid cell lines was confirmed by PCR analysis and Southern blot hybridization. Confirmation of mutant LMP2A protein expression was by immunofluorescence and immunoblotting with a newly identified rat monoclonal antibody that recognizes each of the LMP2A deletion mutations. Lymphoblastoid cell lines infected with recombinant EBV DNAs containing themutations were analyzed for loss of LMP2A's dominant-negative effect on surface immunoglobulin signal transduction by monitoring induction of tyrosine phosphorylation, calcium mobilization, and activation of lyric replication following surface immunoglobulin cross-linking. Domains of LMP2A important for induction of tyrosine phosphorylation, calcium mobilization, and activation of lytic replication were identified.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 16/07/20 alle ore 15:21:19