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Titolo:
COMPLEMENT-MEDIATED KILLING OF PORPHYROMONAS-GINGIVALIS-381 BY THE IMMUNOGLOBULIN-G INDUCED BY RECOMBINANT 40-KDA OUTER-MEMBRANE PROTEIN
Autore:
SAITO S; HAYAKAWA M; HIRATSUKA K; TAKIGUCHI H; ABIKO Y;
Indirizzi:
NIHON UNIV,SCH DENT,DEPT BIOCHEM MATSUDO CHIBA 271 JAPAN
Titolo Testata:
Biochemical and molecular medicine
fascicolo: 2, volume: 58, anno: 1996,
pagine: 184 - 191
SICI:
1077-3150(1996)58:2<184:CKOPBT>2.0.ZU;2-3
Fonte:
ISI
Lingua:
ENG
Soggetto:
BLACK-PIGMENTED BACTEROIDES; GRAM-NEGATIVE BACTERIA; PERIODONTAL-DISEASE; ESCHERICHIA-COLI; CREVICULAR FLUID; HUMAN-SERUM; ANTIBODY; ASACCHAROLYTICUS; IMMUNIZATION; INFECTION;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Science Citation Index Expanded
Citazioni:
40
Recensione:
Indirizzi per estratti:
Citazione:
S. Saito et al., "COMPLEMENT-MEDIATED KILLING OF PORPHYROMONAS-GINGIVALIS-381 BY THE IMMUNOGLOBULIN-G INDUCED BY RECOMBINANT 40-KDA OUTER-MEMBRANE PROTEIN", Biochemical and molecular medicine, 58(2), 1996, pp. 184-191

Abstract

Porphyromonas gingivalis has been implicated as an important pathogenin severe adult periodontitis. We have previously cloned a 40-kDa outer membrane protein from P. gingivalis 381 and succeeded in producing sufficient quantities of the recombinant protein (r40-kDa OMP). r40-kDa OMP has been the subject of considerable interest to us as a possible vaccine candidate. To understand the role of anti-r40-kDa OMP antibody in the host defense mechanisms against P. gingivalis, we examined the involvement of a rabbit antibody against r40-kDa OMP (r40-kDa OMP Ab) to an in vitro complement-mediated bactericidal assay for P. gingivalis 381. By measuring the absorbance values in order to assay the surviving bacteria, we found significant anti-P. gingivalis activity of r40-kDa OMP Ab when guinea pig complement was present. Using affinity-purified immunoglobulin G of r40-kDa OMP Ab (IgG-r40-kDa OMP), we demonstrated that the IgG contributed to anti-P. gingivalis activity in theantibody-complement system. This was effected by measuring the incorporation of tritiated thymidine into newly synthesized nucleic acids. Finally, we confirmed the cell lysis of P. gingivalis 381 exposed to IgG-r40-kDa OMP ill the presence of complement sources in a radioactive bactericidal assay using bacteria labeled with [C-14]sodium acetate. Assembling the data from experiments using component-deficient complements, we concluded that IgG-r40-kDa OMP was related to the killing of P. gingivalis 381 by mediation in the complement activated through boththe classical and the alternative pathways. (C) 1996 Academic Press, Inc.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 27/11/20 alle ore 16:10:50