Catalogo Articoli (Spogli Riviste)

OPAC HELP

Titolo:
SPECIES VARIATION IN THE TESTICULAR ANGIOTENSIN-CONVERTING ENZYME PROMOTER STUDIED IN TRANSGENIC MICE
Autore:
ERICKSON RP; KESSLER S; KREMLING H; SEN GC;
Indirizzi:
UNIV ARIZONA,DEPT PEDIAT,COLL MED,STEELE MEM CHILDRENS RES CTR TUCSONAZ 85724 UNIV ARIZONA,DEPT MOL & CELLULAR BIOL,COLL MED,STEELE MEM CHILDRENS RES CTR TUCSON AZ 85724 CLEVELAND CLIN FDN,RES INST,DEPT MOL BIOL CLEVELAND OH 44195
Titolo Testata:
Molecular reproduction and development
fascicolo: 3, volume: 44, anno: 1996,
pagine: 324 - 331
SICI:
1040-452X(1996)44:3<324:SVITTA>2.0.ZU;2-2
Fonte:
ISI
Lingua:
ENG
Soggetto:
TRANSCRIPTION INITIATION SITES; MOUSE PROTAMINE-1 PROMOTER; REGULATORY ELEMENTS; RIBONUCLEIC-ACID; MESSENGER-RNAS; RAT EPIDIDYMIS; TESTIS; GENE; EXPRESSION; SPERMATOGENESIS;
Keywords:
SPERMATOGENESIS; POSTMEIOTIC GENE EXPRESSION; TRANSCRIPTIONAL REGULATION; PROMOTER ANALYSIS;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Science Citation Index Expanded
Science Citation Index Expanded
Science Citation Index Expanded
Citazioni:
43
Recensione:
Indirizzi per estratti:
Citazione:
R.P. Erickson et al., "SPECIES VARIATION IN THE TESTICULAR ANGIOTENSIN-CONVERTING ENZYME PROMOTER STUDIED IN TRANSGENIC MICE", Molecular reproduction and development, 44(3), 1996, pp. 324-331

Abstract

We have studied the control of transcription of the testicular angiotensin converting enzyme (ACEt) in normal and transgenic mice. Northernanalyses, including a developmental curve and separated germ cells, for ACEt mRNA suggest predominantly post-meiotic expression. Mice transgenic for a construct containing the proximal 298 bp of the rabbit ACEt promoter, with chloramphenicol acetyl transferase (CAT) as a recorder, showed correct tissue regulation while a 86 bp fragment of the promoter led to no expression. Many candidate transacting factor binding elements, previously identified as candidate regulators of transcription driving spermatogenesis, are scattered across this 298 bp in the rabbit (but not the mouse) promoter and may lead to tissue specificity. The recent finding that the proximal 91 bp of the mouse ACEt promoter leads to tissue specific expression of a recorder gene (Howard et al., 1993) emphasizes the difference between the two species and the importance of a cAMP response element (CRE) within this fragment for tissue specific expression. This CRE is conserved in the rabbit promoters we used. (C) 1996 Wiley-Liss, Inc.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 29/09/20 alle ore 07:18:37