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Titolo:
SUPPRESSION OF SYNDECAN-1 EXPRESSION TUMOR-NECROSIS-FACTOR-ALPHA
Autore:
KAINULAINEN V; NELIMARKKA L; JARVELAINEN H; LAATO M; JALKANEN M; ELENIUS K;
Indirizzi:
CHILDRENS HOSP,DEPT SURG,ENDERS 10,300 LONGWOOD AVE BOSTON MA 02115 UNIV TURKU,DEPT SURG TURKU 20520 FINLAND UNIV TURKU,DEPT INTERNAL MED TURKU 20520 FINLAND UNIV TURKU,DEPT BIOCHEM MED TURKU 20520 FINLAND UNIV TURKU,TURKU CTR BIOTECHNOL TURKU 20520 FINLAND
Titolo Testata:
The Journal of biological chemistry
fascicolo: 31, volume: 271, anno: 1996,
pagine: 18759 - 18766
SICI:
0021-9258(1996)271:31<18759:SOSET>2.0.ZU;2-O
Fonte:
ISI
Lingua:
ENG
Soggetto:
HEPARAN-SULFATE PROTEOGLYCANS; MAMMARY EPITHELIAL-CELLS; FIBROBLAST GROWTH-FACTOR; EMBRYONIC TOOTH MESENCHYME; SURFACE PROTEOGLYCAN; ENDOTHELIAL-CELLS; MALIGNANT TRANSFORMATION; VASCULAR ENDOTHELIUM; KAPPA-B; BINDING;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Citazioni:
70
Recensione:
Indirizzi per estratti:
Citazione:
V. Kainulainen et al., "SUPPRESSION OF SYNDECAN-1 EXPRESSION TUMOR-NECROSIS-FACTOR-ALPHA", The Journal of biological chemistry, 271(31), 1996, pp. 18759-18766

Abstract

Syndecan-1 is a cell surface proteoglycan that binds extracellular matrix components and modulates the activity of heparin-binding growth factors, The expression of syndecan-1 is modified during development, carcinogenesis, and tissue regeneration, During cutaneous wound healing, syndecan-1 expression is transiently induced in newly-formed capillaries of granulation tissue as well as in proliferating keratinocytes, To study the mechanisms underlying this regulation we investigated theeffects of several growth factors/cytokines on syndecan-1 expression in two human cell lines: EA.hy 926 endothelial cells and HaCaT keratinocytes. None of these factors significantly altered syndecan-1 mRNA expression in cultured keratinocytes, but when given to endothelial cells, tumor necrosis factor-alpha (TNF-alpha) specifically and dose-dependently suppressed syndecan-1 expression at both mRNA and protein levels, TNF-alpha reduced the amount of syndecan-1 protein in EA.hy 926 cells in both the presence and absence of serum and, at the same time, induced the expression of intercellular adhesion molecule-1 (ICAM-1). The suppressive effect of TNF-alpha on endothelial syndecan-1 expressionwas reproducible in in vivo experiments in which TNF-alpha coated beads were administered directly to healing skin wounds of mice. Data supporting these findings were further obtained by injecting TNF-alpha into an experimental rat granulation tissue model, In this tissue TNF a suppressed syndecan-1 mRNA expression by approximately 80%. These results indicate that TNF-alpha is capable of down-regulating syndecan-1 expression in endothelial cells both in vitro and in vivo and suggest that similar mechanisms may be responsible for the changes in syndecan-1 expression observed during various regenerative, developmental, and malignant processes.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 08/08/20 alle ore 20:05:05