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Titolo:
ASSESSMENT OF A STANDARDIZED REVERSE-TRANSCRIPTASE PCR ASSAY FOR QUANTIFYING HIV-1 RNA IN PLASMA AND SERUM
Autore:
IZOPET J; POGGI C; DUSSAIX E; MANSUY JM; CUBAYNES L; PROFIZI N; LAFEUILLADE A; MARCHOU B; MASSIP P; SAYADA C; PUEL J;
Indirizzi:
CHU TOULOUSE,HOP PURPAN,VIROL LAB F-31059 TOULOUSE FRANCE HOP LA SEYNE,VIROL LAB F-83507 LA SEYNE SUR MER FRANCE CHU BICETRE,VIROL LAB F-94270 LE KREMLIN BICETR FRANCE HOP LA SEYNE SUR MER,SERV MALAD INFECT F-83507 LA SEYNE SUR MER FRANCE CHU TOULOUSE,SERV MALAD INFECT F-31059 TOULOUSE FRANCE ROCHE DIAGNOST SYST F-92521 NEUILLY SUR SEINE FRANCE
Titolo Testata:
Journal of virological methods
fascicolo: 2, volume: 60, anno: 1996,
pagine: 119 - 129
SICI:
0166-0934(1996)60:2<119:AOASRP>2.0.ZU;2-K
Fonte:
ISI
Lingua:
ENG
Soggetto:
HUMAN-IMMUNODEFICIENCY-VIRUS; POLYMERASE CHAIN-REACTION; TYPE-1 RNA; GENE AMPLIFICATION; PROVIRAL DNA; HEPATITIS-C; QUANTITATION; QUANTIFICATION; INFECTION; BLOOD;
Keywords:
HIV-1 RNA; QUANTITATION; RT-PCR;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Science Citation Index Expanded
Science Citation Index Expanded
Citazioni:
30
Recensione:
Indirizzi per estratti:
Citazione:
J. Izopet et al., "ASSESSMENT OF A STANDARDIZED REVERSE-TRANSCRIPTASE PCR ASSAY FOR QUANTIFYING HIV-1 RNA IN PLASMA AND SERUM", Journal of virological methods, 60(2), 1996, pp. 119-129

Abstract

The analytical variability of the new commercially available Reverse Transcriptase-Polymerase Chain Reaction (RT-PCR) assay, Amplicor(TM) HIV-I Monitor(TM), has been assessed to establish criteria for assessing the significance of HIV-I RNA level measurements. Estimations of thestandard deviations (SD) of log-copies in inter-assay (mean 0.09 log)and in inter-laboratory (mean 0.14 log) reproducibility experiments demonstrated that the assay can discriminate with 95% confidence between 3-fold (inter-assay) and 5-fold differences (inter-laboratory). The inter-lot reproducibility (mean 0.10 log) was similar to the inter-assay reproducibility. The HIV-I RNA concentrations measured in plasma collected in potassium EDTA anticoagulant were slightly higher than those measured in plasma collected in sodium citrate. The HIV-1 RNA concentrations measured in sera were about 50%, of the HIV-1 RNA concentrations measured in paired plasma samples. However, there was a strong correlation between these two measurements (P<0.0001). The assay was usedto measure viral RNA in the plasma of 50 HIV-1 positive individuals at different stages of infection. All the individuals had detectable HIV-I RNA (300-957 000 copies/ml). There was no correlation between HIV-I RNA and Immune Complex Dissociated (ICD) p24 antigen, but HIV-I RNA was correlated with CD4+cell counts (P<0.0001) and the clinical stage (P=0.0042), with higher HIV-1 RNA concentrations in patients with a more advanced stage of the disease. The significant association of HIV-1RNA with major markers of HIV infection and the reliability of this sensitive, easy-to-use RT-PCR assay indicate its suitability for use inclinical trials and suggest that this assay is appropriate for routine clinical applications.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 02/12/20 alle ore 05:27:45