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Titolo:
DEGRADATION OF CHYLOMICRON REMNANTS BY MACROPHAGES OCCURS VIA PHAGOCYTOSIS
Autore:
MAMO JCL; ELSEGOOD CL; GENNAT HC; YU K;
Indirizzi:
UNIV WESTERN AUSTRALIA,DEPT MED,STIRLING HIGHWAY NEDLANDS WA 6009 AUSTRALIA UNIV WESTERN AUSTRALIA,DEPT PHYSIOL NEDLANDS WA 6009 AUSTRALIA
Titolo Testata:
Biochemistry
fascicolo: 31, volume: 35, anno: 1996,
pagine: 10210 - 10214
SICI:
0006-2960(1996)35:31<10210:DOCRBM>2.0.ZU;2-N
Fonte:
ISI
Lingua:
ENG
Soggetto:
LOW-DENSITY-LIPOPROTEIN; HUMAN MONOCYTE-MACROPHAGES; BINDING-SITE; CHOLESTEROL DEPOSITION; RECEPTOR; ATHEROSCLEROSIS; PROTEIN; BETA;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Citazioni:
20
Recensione:
Indirizzi per estratti:
Citazione:
J.C.L. Mamo et al., "DEGRADATION OF CHYLOMICRON REMNANTS BY MACROPHAGES OCCURS VIA PHAGOCYTOSIS", Biochemistry, 35(31), 1996, pp. 10210-10214

Abstract

Chylomicron remnants bound to rabbit alveolar macrophages with high-affinity (K-d = 3.3 +/- 0.71 mu g of protein/mL). The binding of chylomicron remnants was competitively inhibited in the presence of unlabeled remnants and to a lesser extent by unlabeled low-density lipoproteins. Pretreatment of cells with either trypsin or pronase inhibited degradation in a dose and time dependent manner, suggesting involvement ofa cell surface protein. Chylomicron remnants were degraded by alveolar macrophages from Watanabe heritable hyperlipidemic (WHHL) rabbits, which are devoid of LDL receptor activity. Moreover, colchicine and monensin which are endocytotic and lysozomal inhibitors, respectively, did not have any effect on the degradation of chylomicron remnants by macrophages from normal rabbits. The absence of divalent cations was found to enhance chylomicron remnant degradation by macrophages. Activated alpha 2-macroglobulin and lactoferrin had no effect on chylomicron remnant degradation, indicating that the low-density lipoprotein receptor-related protein was not involved. in addition, the scavenger receptor inhibitors polyinosinic acid and fucoidan increased degradation of chylomicron remnant-ruling out uptake as a consequence of Lipoprotein modification Rather, the phagocytotic inhibitor cytochalasan D was found to significantly decrease chylomicron remnant degradation. Collectively, our data show that chylomicron remnants are metabolized by phagocytotic pathways initiated after binding to a cell surface protein which is distinct from the LDL receptor, LRP, or scavenger receptors.

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Documento generato il 29/02/20 alle ore 02:06:44