Catalogo Articoli (Spogli Riviste)

OPAC HELP

Titolo:
CRYOPRESERVED HUMAN BONE-MARROW STROMA IS FULLY FUNCTIONAL IN-VITRO
Autore:
NICOL A; NIEDA M; DONALDSON C; DENNINGKENDALL P; TRUMAN C; BRADLEY B; HOWS J;
Indirizzi:
SOUTHMEAD GEN HOSP,DEPT TRANSPLANTAT SCI,SOUTHMEAD RD BRISTOL BS10 5NB AVON ENGLAND UNIV BRISTOL,DEPT TRANSPLANTAT SCI,STEM CELL RES LAB BRISTOL AVON ENGLAND
Titolo Testata:
British Journal of Haematology
fascicolo: 2, volume: 94, anno: 1996,
pagine: 258 - 265
SICI:
0007-1048(1996)94:2<258:CHBSIF>2.0.ZU;2-K
Fonte:
ISI
Lingua:
ENG
Soggetto:
LONG-TERM CULTURE; HEMATOPOIETIC STEM-CELLS; BLOOD PROGENITOR CELLS; UMBILICAL-CORD BLOOD; LIMITING DILUTION; LINES; TRANSPLANTATION; ENGRAFTMENT; RECOVERY;
Keywords:
LONG-TERM CULTURE-INITIATING CELLS; BONE MARROW STROMA; CRYOPRESERVATION; CORD BLOOD; LIMITING DILUTION ASSAY;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Citazioni:
27
Recensione:
Indirizzi per estratti:
Citazione:
A. Nicol et al., "CRYOPRESERVED HUMAN BONE-MARROW STROMA IS FULLY FUNCTIONAL IN-VITRO", British Journal of Haematology, 94(2), 1996, pp. 258-265

Abstract

Human marrow long-term culture (LTC) enables maintenance of both stromal and haemopoietic elements of normal bone marrow (NBM) in vitro for4-6 months. Stroma-based cultures are critical for quantitation of long-term culture initiating cells (LTC-IC), the most primitive human haemopoietic cells measurable in vitro. Supply of NBM can be sporadic, and up to 3-4 weeks in culture is required for stromal maturity. Stromaavailability for experimental purposes can therefore be limited. Efforts to produce transformed human and transfected murine stromal cell lines comparable to NBM stroma have had some success, As an alternative, we investigated cryopreserved NBM and cryopreserved preformed stroma. Function of cryopreserved and control fresh NBM stroma was similar when evaluated for up to 12 weeks in LTC, We have also demonstrated that stroma derived from cryopreserved NBM or preformed cryopreserved NBMstroma can sustain third-party haemopoiesis as efficiently as fresh NBM stroma in LTC, Batched cryopreserved stroma is a convenient, rapidly available, source of functional stroma which avoids the logistic difficulties and lack of standardization associated with stroma from fresh NBM. This important advance will enhance the use of stroma-based LTCin studies of human haemopoiesis.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 18/09/20 alle ore 09:09:50