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Titolo:
CYTOTOXICITY OF PERITONEAL MURINE MACROPHAGES AGAINST ENCAPSULATED PANCREATIC RAT ISLETS - IN-VIVO AND IN-VITRO STUDIES
Autore:
KESSLER L; JESSER C; LOMBARD Y; KARSTEN V; BELCOURT A; PINGET M; POINDRON P;
Indirizzi:
HOP UNIV STRASBOURG,JEUNE EQUIPE MED UNIV,SERV ENDOCRINOL,PAVILLON LERICHE F-67091 STRASBOURG FRANCE FAC PHARM,LAB IMMUNOPHARMACOL STRASBOURG FRANCE
Titolo Testata:
Journal of leukocyte biology
fascicolo: 6, volume: 60, anno: 1996,
pagine: 729 - 736
SICI:
0741-5400(1996)60:6<729:COPMMA>2.0.ZU;2-N
Fonte:
ISI
Lingua:
ENG
Soggetto:
DIABETIC MICE; TRANSPLANTATION; XENOGRAFTS; INSULIN; CELLS; MICROCAPSULES; NICOTINAMIDE; LANGERHANS; PROTECTION; CYTOKINES;
Keywords:
CYTOKINE; BIOARTIFICIAL PANCREAS; ARTIFICIAL MEMBRANE; TRANSPLANTATION;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Science Citation Index Expanded
Citazioni:
29
Recensione:
Indirizzi per estratti:
Citazione:
L. Kessler et al., "CYTOTOXICITY OF PERITONEAL MURINE MACROPHAGES AGAINST ENCAPSULATED PANCREATIC RAT ISLETS - IN-VIVO AND IN-VITRO STUDIES", Journal of leukocyte biology, 60(6), 1996, pp. 729-736

Abstract

The aim of this work was to study in vivo and in vitro the involvement of macrophages and interleukin-1 beta (IL-1 beta) in the necrosis ofencapsulated islets during xenograft and to evaluate the immunoprotective efficiency of the AN69 membrane, In vivo, 6 days after implantation, 65% of the membrane surface of the devices containing the islets was colonized with macrophages compared with only 5% of the surface of the empty control devices, The morphological aspect of implanted islets was altered and their insulin release decreased significantly compared with freshly isolated ones (265 +/- 50 vs. 501 +/- 81 mu U/mL), In vitro, the insulin release of encapsulated islets cultured for 2 days decreased to 32 and 28%, respectively, in the presence of IL-1 beta and macrophages. The addition of anti-IL-1 beta antibody to the co-culture of macrophages and islets did not modify this loss of functional activity, Furthermore, IL-1 beta passed through the AN69 membrane, In conclusion, macrophages are involved in damaging encapsulated pancreaticislets and are probably partly responsible for islet transplantation failure.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 02/12/20 alle ore 05:21:30