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Titolo:
PROPERTIES OF WNT-1 PROTEIN THAT ENABLE CELL-SURFACE ASSOCIATION
Autore:
SCHRYVER B; HINCK L; PAPKOFF J;
Indirizzi:
MEGABIOS CORP,863A MITTEN RD BURLINGAME CA 94010 STANFORD UNIV,CANC BIOL PROGRAM STANFORD CA 94305 SUGEN INC REDWOOD CITY CA 94063
Titolo Testata:
Oncogene
fascicolo: 2, volume: 13, anno: 1996,
pagine: 333 - 342
SICI:
0950-9232(1996)13:2<333:POWPTE>2.0.ZU;2-A
Fonte:
ISI
Lingua:
ENG
Soggetto:
FIBROBLAST GROWTH-FACTOR; MAMMARY ONCOGENE INT-1; HEPARAN-SULFATE; EXTRACELLULAR-MATRIX; EPITHELIAL-CELLS; WINGLESS PROTEIN; EXPRESSION; DROSOPHILA; BINDING; TRANSFORMATION;
Keywords:
WNT-1; BASIC SEQUENCE MOTIF; PROTEOGLYCAN;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Science Citation Index Expanded
Science Citation Index Expanded
Citazioni:
52
Recensione:
Indirizzi per estratti:
Citazione:
B. Schryver et al., "PROPERTIES OF WNT-1 PROTEIN THAT ENABLE CELL-SURFACE ASSOCIATION", Oncogene, 13(2), 1996, pp. 333-342

Abstract

Members of the highly conserved Wnt gene family serve key functions in cellular growth and differentiation. Regulated expression of the murine Wnt-1 protooncogene is essential for normal development of the embryonic nervous system and when misexpressed in the mammary gland leadsto hyperplasias and tumorigenic progression. Wnt-1 encodes a cysteinerich glycoprotein that is secreted and tightly associated with the cell surface and/or extracellular matrix. This restricted diffusion of Wnt-1 protein is important for specifying a spatially defined pattern of Wnt-activity necessary for cell to cell signaling in cell growth anddifferentiation. Since the receptors and cell surface molecules that bind Wnt proteins are as yet unknown we have sought to define the sequences within Wnt-1 protein that enable cell surface association and todefine the nature of the cell surface structures with which Wnt-1 protein associates. First, site directed mutagenesis was used to identifya basic amino acid sequence motif within Wnt-1 protein that is required for this protein to accumulate on the cell surface. Second, wild type Wnt-1 protein was expressed in a series of proteoglycan-deficient CHO cell lines to show that, contrary to expectations, cellular glycosaminoglycans are not required for processing, secretion, cell surface association and accumulation of Wnt-1 protein.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 27/11/20 alle ore 21:58:34