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Titolo:
CPP32 INHIBITION PREVENTS FAS-INDUCED CERAMIDE GENERATION AND APOPTOSIS IN HUMAN-CELLS
Autore:
GAMEN S; MARZO I; ANEL A; PINEIRO A; NAVAL J;
Indirizzi:
UNIV ZARAGOZA,FAC CIENCIAS,DEPT BIOQUIM & BIOL MOLEC & CELULAR E-50009 ZARAGOZA SPAIN UNIV ZARAGOZA,FAC CIENCIAS,DEPT BIOQUIM & BIOL MOLEC & CELULAR E-50009 ZARAGOZA SPAIN
Titolo Testata:
FEBS letters
fascicolo: 2, volume: 390, anno: 1996,
pagine: 233 - 237
SICI:
0014-5793(1996)390:2<233:CIPFCG>2.0.ZU;2-P
Fonte:
ISI
Lingua:
ENG
Soggetto:
TUMOR-NECROSIS-FACTOR; ACTIVATED PROTEIN-KINASE; INTERLEUKIN-1-BETA CONVERTING-ENZYME; POLY(ADP-RIBOSE) POLYMERASE; ICE/CED-3 PROTEASE; DNA FRAGMENTATION; DEATH; ICE; CYTOTOXICITY; MONOCYTES;
Keywords:
FAS; TUMOR NECROSIS FACTOR ALPHA; CERAMIDE; ICE-LIKE PROTEASES; APOPTOSIS;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Science Citation Index Expanded
Citazioni:
34
Recensione:
Indirizzi per estratti:
Citazione:
S. Gamen et al., "CPP32 INHIBITION PREVENTS FAS-INDUCED CERAMIDE GENERATION AND APOPTOSIS IN HUMAN-CELLS", FEBS letters, 390(2), 1996, pp. 233-237

Abstract

Intracellular activation of sphingomyelinase, leading to ceramide generation, and ICE-like proteases have been implicated in TNF and Fas-induced apoptosis, but the links between these intracellular apoptotic mediators remain undefined, We show here that a specific peptide inhibitor of the ICE-like protease CPP32/Yama (DEVD-CHO) blocks anti-Fas-induced apoptosis in Jurkat and U937 cells, while having no effect on TNF-induced apoptosis in U937 cells. This peptide also prevents ceramide accumulation induced by Pas engagement, Jurkat and U937 cells, as web as their mtDNA-depleted derived lines (rho degrees cells), were sensitive to ceramide toxicity, which was not prevented by ICE-like proteaseinhibitors. These results, taken together, suggest that ICE-like protease activation is a prerequisite for ceramide generation and subsequent apoptosis, at least in the case of Fas-induced cell death.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 04/12/20 alle ore 22:16:49