Catalogo Articoli (Spogli Riviste)

OPAC HELP

Titolo:
ACTIVATION OF PROTEIN-KINASE C-ALPHA AND TRANSLOCATION OF THE MYRISTOYLATED ALANINE-RICH C-KINASE SUBSTRATE CORRELATE WITH PHORBOL ESTER-ENHANCED NORADRENALINE RELEASE FROM SH-SY5Y HUMAN NEUROBLASTOMA-CELLS
Autore:
GOODALL AR; TURNER NA; WALKER JH; BALL SG; VAUGHAN PFT;
Indirizzi:
UNIV LEEDS,INST CARDIOVASC RES LEEDS LS2 9JT W YORKSHIRE ENGLAND UNIV LEEDS,INST CARDIOVASC RES LEEDS LS2 9JT W YORKSHIRE ENGLAND UNIV LEEDS,DEPT BIOCHEM & MOL BIOL LEEDS LS2 9JT W YORKSHIRE ENGLAND
Titolo Testata:
Journal of neurochemistry
fascicolo: 1, volume: 68, anno: 1997,
pagine: 392 - 401
SICI:
0022-3042(1997)68:1<392:AOPCAT>2.0.ZU;2-A
Fonte:
ISI
Lingua:
ENG
Soggetto:
NEUROTRANSMITTER RELEASE; PLASMA-MEMBRANE; H-3 NORADRENALINE; DOWN-REGULATION; MARCKS PROTEIN; ACTIN FILAMENT; EVOKED RELEASE; INITIAL RATE; PHOSPHORYLATION; LOCALIZATION;
Keywords:
MYRISTOYLATED ALANINE-RICH C-KINASE SUBSTRATE; NORADRENALINE RELEASE; PHORBOL ESTER; PROTEIN KINASE C-ALPHA; SH-SY5Y NEUROBLASTOMA CELLS; TRANSLOCATION;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Science Citation Index Expanded
Citazioni:
38
Recensione:
Indirizzi per estratti:
Citazione:
A.R. Goodall et al., "ACTIVATION OF PROTEIN-KINASE C-ALPHA AND TRANSLOCATION OF THE MYRISTOYLATED ALANINE-RICH C-KINASE SUBSTRATE CORRELATE WITH PHORBOL ESTER-ENHANCED NORADRENALINE RELEASE FROM SH-SY5Y HUMAN NEUROBLASTOMA-CELLS", Journal of neurochemistry, 68(1), 1997, pp. 392-401

Abstract

The aim of this study was to investigate the mechanism by which short-term pretreatment with the phorbol ester 12-O-tetradecanoylphorbol 13-acetate (TPA; 100 nM) enhances noradrenaline (NA) release from the human neuroblastoma cell line SH-SY5Y. Subcellular fractionation and immunocytochemical studies demonstrated that an 8-min TPA treatment caused translocation of the alpha-subtype of protein kinase C (PKC) from the cytosol to the plasma membrane, In contrast, TPA altered the distribution of PKC-epsilon from cytosolic and membrane-associated to cytoskeleton- and membrane-associated. TPA had no effect on the cytosolic location of PKC-zeta. Subcellular fractionation studies also showed that the myristoylated alanine-rich C-kinase substrate (MARCKS), a major neuronal PKC substrate that has been implicated in the mechanism of neurotransmitter release, translocated from membranes to cytosol in response to an 8-min TPA treatment, Under these conditions the level of phosphorylation of MARCKS increased threefold. The ability of TPA to enhance NA release and to cause the translocation and phosphorylation of MARCKS was inhibited by the PKC inhibitor Ro 31-8220 (10 mu M). Selective down-regulation of PKC subtypes by prolonged exposure to phorbol 12,13-dibutyrate (100 nM) attenuated the TPA-induced enhancement of NA release and the translocation of MARCKS over an interval similar to thatof down-regulation of PKC-alpha (but not -epsilon or -zeta), Thus, wehave demonstrated a strong correlation between the translocation of MARCKS and the enhancement of NA release from SH-SY5Y cells due to the TPA-induced activation of PKC-alpha.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 03/04/20 alle ore 06:56:42