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Titolo:
DNA-SEQUENCE ANALYSIS OF HPRT MUTATIONS IN LYMPHOCYTES FROM SPRAGUE-DAWLEY ROTS TREATED WITH 7,12-DIMETHYLBENZ[A]ANTHRACENE
Autore:
HEFLICH RH; MITTELSTAEDT RA; MANJANATHA MG; LYNCOOK LE; AIDOO A;
Indirizzi:
NATL CTR TOXICOL RES,DIV GENET TOXICOL,HFT-200,3900 NCTR RD JEFFERSONAR 72079
Titolo Testata:
Environmental and molecular mutagenesis
fascicolo: 1, volume: 28, anno: 1996,
pagine: 5 - 12
SICI:
0893-6692(1996)28:1<5:DAOHMI>2.0.ZU;2-Y
Fonte:
ISI
Lingua:
ENG
Soggetto:
ETHYL-N-NITROSOUREA; HAMSTER OVARY CELLS; GRADIENT GEL-ELECTROPHORESIS; HA-RAS ONCOGENE; MOUSE SKIN; IN-VIVO; MOLECULAR ANALYSIS; MAMMARY CARCINOGENESIS; FISCHER-344 RATS; INVIVO EXPOSURE;
Keywords:
DENATURING GRADIENT GEL ELECTROPHORESIS; 7,12-DIMETHYLBENZ[A]ANTHRACENE; SPRAGUE-DAWLEY RAT; LYMPHOCYTES; HPRT; MUTATION;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Science Citation Index Expanded
Citazioni:
54
Recensione:
Indirizzi per estratti:
Citazione:
R.H. Heflich et al., "DNA-SEQUENCE ANALYSIS OF HPRT MUTATIONS IN LYMPHOCYTES FROM SPRAGUE-DAWLEY ROTS TREATED WITH 7,12-DIMETHYLBENZ[A]ANTHRACENE", Environmental and molecular mutagenesis, 28(1), 1996, pp. 5-12

Abstract

Treatment of female Sprague-Dawley rats with the potent mammary glandcarcinogen 7,12-dimethylbenz[a]anthracene (DMBA) results in the formation of DNA adducts with dG and dA and in the induction of 6-thioguanine-resistant (TG(r)) lymphocyte mutants. In this study, we have examined the types of mutations induced in TG(r) lymphocytes from DMBA-treated rats. DNA from 263 TG(r) lymphocyte clones was screened for mutations in exons 2, 3, and 8 of the hprt gene by polymerase chain reaction (PCR) amplification of the exons followed by heteroduplex analysis using denaturing gradient-gel electrophoresis. Twenty-five of the clones produced heteroduplexes in exon 2, 35 produced heteroduplexes in exon 3, and 36 produced heteroduplexes in exon 8. Direct sequence analysis of the heteroduplexes revealed 96 mutations, and at least 74 of these mutations were produced independently. Eighty-five of the total mutations were simple bose pair (bp) substitutions, with A --> T and G --> Ttransversions being the predominant types. Seven mutations were deletions, three were complex bp substitutions, and one was an insertion. The results suggest that the types of mutations produced by DMBA in ratlymphocytes are specific to the DNA adducts produced by this compound. (C) 1996 Wiley-Liss, Inc.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 07/07/20 alle ore 20:57:18