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Titolo:
IN-VITRO EFFECTS OF ANTI-HIV IMMUNOTOXINS DIRECTED AGAINST MULTIPLE EPITOPES ON HIV TYPE-1 ENVELOPE GLYCOPROTEIN-160
Autore:
PINCUS SH; WEHRLY K; COLE R; FANG H; LEWIS GK; MCCLURE J; CONLEY AJ; WAHREN B; POSNER MR; NOTKINS AL; TILLEY SA; PINTER A; EIDEN L; TEINTZE M; DORWARD D; TOLSTIKOV VV;
Indirizzi:
NIAID,ROCKY MT LABS,MICROBIAL STRUCT & FUNCT LAB,903 S 4TH ST HAMILTON MT 59840 NIAID,ROCKY MT LABS,PERSISTENT VIRAL DIS LAB HAMILTON MT 59840 NIAID,ROCKY MT LABS,ELECTRON MICROSCOPY BRANCH HAMILTON MT 59840 UNIV MARYLAND,SCH MED BALTIMORE MD 20201 BRISTOL MYERS SQUIBB PHARMACEUT RES INST SEATTLE WA 98121 MERCK SHARP & DOHME LTD,RES LABS W POINT PA 19486 KAROLINSKA INST S-105 STOCKHOLM SWEDEN NEW ENGLAND DEACONESS HOSP BOSTON MA 02215 NIDR,NIH BETHESDA MD 20892 NIMH,NIH BETHESDA MD 20892 PUBL HLTH RES INST NEW YORK NY 10016 MONTANA STATE UNIV BOZEMAN MT 59717
Titolo Testata:
AIDS research and human retroviruses
fascicolo: 11, volume: 12, anno: 1996,
pagine: 1041 - 1051
SICI:
0889-2229(1996)12:11<1041:IEOAID>2.0.ZU;2-Q
Fonte:
ISI
Lingua:
ENG
Soggetto:
HUMAN-IMMUNODEFICIENCY-VIRUS; RICIN-A-CHAIN; HUMAN MONOCLONAL-ANTIBODY; INDUCED SYNCYTIUM FORMATION; EXOTOXIN HYBRID PROTEIN; RECOMBINANT SOLUBLE CD4; CD4-PSEUDOMONAS EXOTOXIN; INFECTED-CELLS; T-CELLS; PEPTIDE DERIVATIVES;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Science Citation Index Expanded
Citazioni:
57
Recensione:
Indirizzi per estratti:
Citazione:
S.H. Pincus et al., "IN-VITRO EFFECTS OF ANTI-HIV IMMUNOTOXINS DIRECTED AGAINST MULTIPLE EPITOPES ON HIV TYPE-1 ENVELOPE GLYCOPROTEIN-160", AIDS research and human retroviruses, 12(11), 1996, pp. 1041-1051

Abstract

We have used a panel of anti-gp160 MAbs to construct anti-HIV immunotoxins by coupling antibodies to ricin A chain (RAG), The ability of the immunotoxins to kill HIV-1-infected cells and halt the spread of infection was tested in tissue culture on persistently and acutely infected cell lines and primary lymphocyte cultures stimulated with phytohemagglutinin (PHA blasts), Laboratory strains and clinical isolates of HIV both were tested, The constitution and antigen-binding capacity of the immunotoxins mere confirmed by ELISA and indirect immunofluorescence. Immunotoxins that bind epitopes exposed on the cell surface effectively killed persistently infected cells, although killing was not directly proportional to binding of immunotoxin to cell. The activity of anti-gp41, but not anti-gp120, immunotoxins was markedly enhanced in the presence of soluble CD4 or peptides corresponding to the CDR3 region of CD4, CD4-mediated enhancement of anti-gp41 immunotoxin activity was observed for laboratory strains neutralized by sCD4 and for clinical isolates that were resistant to neutralization by sCD4, Immunotoxin action was potentiated by brefeldin A, bafilomycin A1, cortisone, and an amphipathic fusion peptide, but not by cytochalasin D, nocodazol, monodansyl cadaverine, or trans-retinoic acid. Anti-HIV immunotoxins are useful tools with which to study the functional expression of gp120/gp41 antigens on the surface of HIV-infected cells, as well as potential AIDS therapeutics, Because these studies relate to the accessibility of viral antigens to antibody-mediated attack, these studies also have relevance for vaccine development.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 01/12/20 alle ore 09:20:19