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Titolo:
REGIONAL DISTRIBUTION AND TIME-COURSE OF CALPAIN ACTIVATION FOLLOWINGKAINATE-INDUCED SEIZURE ACTIVITY IN ADULT-RAT BRAIN
Autore:
BI XN; CHANG V; SIMAN R; TOCCO G; BAUDRY M;
Indirizzi:
UNIV SO CALIF,NEUROSCI PROGRAM,HNB 311 LOS ANGELES CA 90089 CEPHALON INC W CHESTER PA 19380
Titolo Testata:
Brain research
fascicolo: 1-2, volume: 726, anno: 1996,
pagine: 98 - 108
SICI:
0006-8993(1996)726:1-2<98:RDATOC>2.0.ZU;2-P
Fonte:
ISI
Lingua:
ENG
Soggetto:
HIPPOCAMPAL SLICES; SYNAPTIC TRANSMISSION; SPECTRIN DEGRADATION; DENTATE GYRUS; KAINIC ACID; RECEPTOR; PROTEOLYSIS; ISCHEMIA; NEURONS; DAMAGE;
Keywords:
SEIZURE; CALPAIN; SPECTRIN; GLUTAMATE; HIPPOCAMPUS; NEUROPATHOLOGY;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Citazioni:
29
Recensione:
Indirizzi per estratti:
Citazione:
X.N. Bi et al., "REGIONAL DISTRIBUTION AND TIME-COURSE OF CALPAIN ACTIVATION FOLLOWINGKAINATE-INDUCED SEIZURE ACTIVITY IN ADULT-RAT BRAIN", Brain research, 726(1-2), 1996, pp. 98-108

Abstract

Systemic injection of kainic acid (KA) in adult rat elicits a patternof neuronal pathology which exhibits several features of human temporal lobe epilepsy, KA-induced seizure activity is accompanied by the activation of the calcium-dependent protease calpain in limbic structures. In the present study, we evaluated the spatio-temporal activation of calpain after the onset of seizure activity by immunohistochemistry using an antibody for the spectrin breakdown product (sbdp) generated by calpain-mediated spectrin proteolysis. In addition, we compared thechanges in sbdp immunoreactivity with those in immunoreactivity to subunits of the Glu/AMPA receptors (GluR(1) and GluR(2/3)). One hour after seizure onset, sbdp accumulation was observed in selected interneurons in stratum oriens and in the hilus of the dentate gyrus. By 4 h, sbdp immunoreactivity was prominent in dendritic fields of the hippocampus as well as in neurons in thalamus and piriform cortex. By 8 h, sbdp immunoreactivity had disappeared from interneurons but was localizedin pyramidal eel bodies in hippocampus. Intense labeling of cell bodies and dendritic fields persisted until 5 days following KA treatment. Changes in GluR subunit immunoreactivity were mirror images of those seen for sbdp. In general, increased sbdp immunoreactivity in deodritic fields was associated with decreased GluR(1) immunoreactivity. However, increased sbdp immunoreactivity in neuronal perikarya was also associated with increased GluR immunoreactivity, These results indicate that calpain activation following seizure onset exhibits a specific spatio-temporal pattern, with activation in restricted interneurons preceding widespread activation in pyramidal neurons. Calpain activation also precedes neuronal pathology and could thus represent an initial trigger for neuronal pathology. Finally, the results suggest that calpainactivation produces rapid alterations in GluR subunit properties which could be involved in the hyperexcitability observed following seizure activity.

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Documento generato il 18/01/21 alle ore 15:33:23