Catalogo Articoli (Spogli Riviste)

OPAC HELP

Titolo:
ELASTASE, BUT NOT PROTEINASE-3 (PR3), INDUCES PROTEINURIA ASSOCIATED WITH LOSS OF GLOMERULAR-BASEMENT-MEMBRANE HEPARAN-SULFATE AFTER IN-VIVO RENAL PERFUSION IN RATS
Autore:
HEERINGA P; VANDENBORN J; BROUWER E; DOLMAN KM; KLOK PA; HUITEMA MG; LIMBURG PC; BAKKER MAH; BERDEN JHM; DAHA MR; KALLENBERG CGM;
Indirizzi:
UNIV GRONINGEN HOSP,DEPT CLIN IMMUNOL,OOSTERSINGEL 59 NL-9713 EZ GRONINGEN NETHERLANDS UNIV GRONINGEN HOSP,DEPT CLIN IMMUNOL NL-9713 EZ GRONINGEN NETHERLANDS UNIV GRONINGEN HOSP,DEPT RHEUMATOL NL-9713 EZ GRONINGEN NETHERLANDS UNIV GRONINGEN HOSP,DEPT PATHOL NL-9713 EZ GRONINGEN NETHERLANDS NETHERLANDS RED CROSS,BLOOD TRANSFUS SERV,CENT LAB,DEPT IMMUNOL HAEMATOL AMSTERDAM NETHERLANDS UNIV NIJMEGEN HOSP,DEPT NEPHROL NIJMEGEN NETHERLANDS LEIDEN UNIV HOSP,DEPT NEPHROL NL-2333 AA LEIDEN NETHERLANDS
Titolo Testata:
Clinical and experimental immunology
fascicolo: 2, volume: 105, anno: 1996,
pagine: 321 - 329
SICI:
0009-9104(1996)105:2<321:EBNP(I>2.0.ZU;2-W
Fonte:
ISI
Lingua:
ENG
Soggetto:
POLYMORPHONUCLEAR LEUKOCYTE; WEGENERS GRANULOMATOSIS; INCREASED PERMEABILITY; SULFATE PROTEOGLYCAN; NEUTRAL PROTEINASES; LYSOSOMAL-ENZYMES; HUMAN-NEUTROPHILS; DEGRADATION; GLOMERULONEPHRITIS; AUTOANTIBODIES;
Keywords:
SERINE PROTEASES; NEUTROPHILS; GLOMERULONEPHRITIS; EXTRACELLULAR MATRIX; ISOELECTRIC POINT;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Citazioni:
34
Recensione:
Indirizzi per estratti:
Citazione:
P. Heeringa et al., "ELASTASE, BUT NOT PROTEINASE-3 (PR3), INDUCES PROTEINURIA ASSOCIATED WITH LOSS OF GLOMERULAR-BASEMENT-MEMBRANE HEPARAN-SULFATE AFTER IN-VIVO RENAL PERFUSION IN RATS", Clinical and experimental immunology, 105(2), 1996, pp. 321-329

Abstract

Elastase, but not PR3, induces proteinuria associated with loss of glomerular basement membrane (GEM) heparan sulphate after in vivo renal perfusion in rats. PR3 and elastase are cationic neutral serine proteinases present in the azurophilic granules of polymorphonuclear leucocytes. Release of these proteolytic enzymes along the glomerular capillary wall may induce glomerular injury. Here, we investigated the effects of PR3 and elastase on the induction of proteinuria and glomerular injury after renal perfusion of these enzymes in Brown-Norway rats. Perfusion of active elastase induced a dose-dependent proteinuria 24h after perfusion, while inactivated elastase did not. Perfusion of comparable amounts of active PR3 did not induce proteinuria. Light and electron microscopy showed no morphological abnormalities in any experimental group. However, immunohistology revealed that proteinuria occurring after perfusion of active elastase was associated with a strong reduction in intraglomerular expression of the heparan sulphate side chain and, to a lesser extent, of the protein core of heparan sulphate proteoglycans (HSPG). In vitro, both elastase and PR3 digested HSPG. However, PR3 bound to a lesser extent to HSPG than elastase. We conclude thatelastase, but not PR3, induces proteinuria after in vivo renal perfusion. This differential effect probably relates to different binding tothe GBM of those enzymes due to differences in their isoelectric points. Degradation of heparan sulfate proteoglycans, leading to the disappearance of their side chains that contribute to the polyanionic structure of the GBM, appears to be involved in the induction of proteinuria after perfusion of elastase.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 04/12/20 alle ore 13:07:33