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Titolo:
AN IMMUNOCYTOCHEMICAL STUDY OF THE DISTRIBUTION OF AMPA SELECTIVE GLUTAMATE-RECEPTOR SUBUNITS IN THE NORMAL HUMAN MOTOR SYSTEM
Autore:
WILLIAMS TL; INCE PG; OAKLEY AE; SHAW PJ;
Indirizzi:
UNIV NEWCASTLE UPON TYNE,ROYAL VICTORIA INFIRM,DEPT NEUROL,WARD 11,QUEEN VICTORIA RD NEWCASTLE TYNE NE1 4LP TYNE & WEAR ENGLAND UNIV NEWCASTLE UPON TYNE,DIV CLIN NEUROSCI NEWCASTLE TYNE NE1 7RU TYNE & WEAR ENGLAND UNIV NEWCASTLE UPON TYNE,DEPT NEUROPATHOL NEWCASTLE TYNE NE1 7RU TYNE& WEAR ENGLAND UNIV NEWCASTLE UPON TYNE,MRC,NEUROCHEM PATHOL UNIT NEWCASTLE TYNE NE17RU TYNE & WEAR ENGLAND
Titolo Testata:
Neuroscience
fascicolo: 1, volume: 74, anno: 1996,
pagine: 185 - 198
SICI:
0306-4522(1996)74:1<185:AISOTD>2.0.ZU;2-3
Fonte:
ISI
Lingua:
ENG
Soggetto:
AMINO-ACID RECEPTORS; RAT SPINAL-CORD; CENTRAL-NERVOUS-SYSTEM; BINDING-SITES; DIFFERENTIAL EXPRESSION; MESSENGER-RNAS; KAINIC ACID; ANTIPEPTIDE ANTIBODIES; NEURONAL DEGENERATION; NEUROLOGICAL DISEASE;
Keywords:
AMYOTROPHIC LATERAL SCLEROSIS; SELECTIVE VULNERABILITY; MOTOR NEURON; NON-NMDA;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Citazioni:
72
Recensione:
Indirizzi per estratti:
Citazione:
T.L. Williams et al., "AN IMMUNOCYTOCHEMICAL STUDY OF THE DISTRIBUTION OF AMPA SELECTIVE GLUTAMATE-RECEPTOR SUBUNITS IN THE NORMAL HUMAN MOTOR SYSTEM", Neuroscience, 74(1), 1996, pp. 185-198

Abstract

Glutamate is the major mediator of fast excitatory neurotransmission in the mammalian central nervous system. Disturbances of this neurotransmitter system have been implicated in chronic degenerative neurological disease. Recently, major advances in our knowledge and understanding of the molecular biology of the glutamatergic receptor system have been made. It is now known that functional glutamate receptors consistof various combinations of some 20 identified subunits. A growing body of circumstantial evidence suggests that the non-N-methyl-D-aspartate subtype of glutamate receptors may mediate, at least in part, the selective motor neuron death seen in the human neurodegenerative diseaseamyotrophic lateral sclerosis. We have used subunit specific immunocytochemistry to study the distribution and potential subunit composition of the pha-amino-3-hydroxy-5-methyl-4-isoxazole-propionic acid (AMPA) selective glutamate receptors, (a subgroup of non-N-methyl-D-aspartate selective glutamate receptors formed by combinations of GluR1-4 subunits), in the human motor system. Motor neurons in the spinal cord, brainstem, and motor cortex were relatively strongly immunoreactive with the GluR2/3 subunit antibody, moderately so with the GluR4 subunit antibody, and showed relatively low levels of immunoreactivity with theGluR1 subunit antibody. This is the first detailed study of AMPA receptor subunit expression in the human motor system. Motor neurons express a distinct subunit profile when compared with other groups of neurons in the human nervous system. There were no significant differences in the pattern of relative AMPA subunit expression (GluR2/3 greater than or equal to GluR4 > GluR1) between groups of motor neurons typically affected (in the spinal cord and hypoglossal nucleus), or spared (oculomotor and Onuf's nucleus) by the amyotrophic lateral sclerosis disease process. However, oculomotor motor neurons had higher levels of expression of all AMPA subunit proteins which may indicate greater AMPA mediated glutamatergic input in the normal function of this neuronal population. This study does not support a role for differential subunitcomposition of AMPA receptors in determining the selective vulnerability of motor neurons in amyotrophic lateral sclerosis. However, the overall density of receptors may be of importance. Copyright (C) 1996 IBRO.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 18/09/20 alle ore 18:45:36