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Titolo:
THE USE OF COMPOUND HETEROZYGOTES AND HPRT SELECTION TO ANALYZE X-LINKED MOTTLED ALLELES ASSOCIATED WITH PRENATAL LETHALITY
Autore:
MASSON W; HOLT S; REED V; BOYD Y;
Indirizzi:
MRC,MAMMALIAN GENET UNIV,DIV GENET HARWELL OX11 0RD OXON ENGLAND MRC,MAMMALIAN GENET UNIV,DIV GENET HARWELL OX11 0RD OXON ENGLAND
Titolo Testata:
Mammalian genome
fascicolo: 7, volume: 7, anno: 1996,
pagine: 486 - 489
SICI:
0938-8990(1996)7:7<486:TUOCHA>2.0.ZU;2-B
Fonte:
ISI
Lingua:
ENG
Soggetto:
MENKES DISEASE GENE; COPPER-TRANSPORTING ATPASE; OCCIPITAL HORN SYNDROME; MURINE HOMOLOG; CANDIDATE GENE; MUTATIONS; MOUSE; MICE; LOCUS; MNK;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Science Citation Index Expanded
Science Citation Index Expanded
Citazioni:
19
Recensione:
Indirizzi per estratti:
Citazione:
W. Masson et al., "THE USE OF COMPOUND HETEROZYGOTES AND HPRT SELECTION TO ANALYZE X-LINKED MOTTLED ALLELES ASSOCIATED WITH PRENATAL LETHALITY", Mammalian genome, 7(7), 1996, pp. 486-489

Abstract

X-linked mutant alleles associated with prenatal male lethality are difficult to analyze because only heterozygous females are readily available for study. Genomic analysis of the mutant allele is facilitated by the construction of somatic cell hybrids because this enables the segregation of the X Chromosomes (Chrs) that carry the mutant and wild-type alleles. We describe here a method that ensures that the X Chr carrying the mutant allele is retained in somatic cell hybrids in an active selectable state. This is achieved by mating heterozygous females to males that carry a mutation at the hypoxanthine phosphoribosyl transferase (Hprt) locus. The resultant F-1 females are compound heterozygotes, and when cells from these females are fused to HPRT-Chinese hamster cells and subjected to selection in HAT medium, the only survivorsare those hybrid cells that retain an active X Chr carrying the mutant allele together with the wild-type Hprt allele. We use hybrids constructed by this method to demonstrate that there are no gross deletionsor genomic rearrangements present in three mottled alleles associatedwith prenatal male lethality.

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Documento generato il 29/11/20 alle ore 16:27:12