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Titolo:
APOPTOSIS IN HUMAN MONOCYTE-MACROPHAGES EXPOSED TO OXIDIZED LOW-DENSITY-LIPOPROTEIN
Autore:
HARDWICK SJ; HEGYI L; CLARE K; LAW NS; CARPENTER KLH; MITCHINSON MJ; SKEPPER JN;
Indirizzi:
UNIV CAMBRIDGE,DEPT PATHOL,DIV CELLULAR PATHOL,TENNIS COURT RD CAMBRIDGE CB2 1QP ENGLAND UNIV CAMBRIDGE,DEPT ANAT,MULTIIMAGING CTR CAMBRIDGE CB2 3DY ENGLAND
Titolo Testata:
Journal of pathology
fascicolo: 3, volume: 179, anno: 1996,
pagine: 294 - 302
SICI:
0022-3417(1996)179:3<294:AIHMET>2.0.ZU;2-W
Fonte:
ISI
Lingua:
ENG
Soggetto:
PROGRAMMED CELL-DEATH; LIPID-PEROXIDATION; DNA FRAGMENTATION; PROTEIN-SYNTHESIS; CYTO-TOXICITY; PROLIFERATION; ATHEROSCLEROSIS; IDENTIFICATION; CHOLESTEROL; INDUCTION;
Keywords:
OXIDIZED LDL; APOPTOSIS; CYTOXICITY; MONOCYTE-MACROPHAGES (HUMAN);
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Citazioni:
35
Recensione:
Indirizzi per estratti:
Citazione:
S.J. Hardwick et al., "APOPTOSIS IN HUMAN MONOCYTE-MACROPHAGES EXPOSED TO OXIDIZED LOW-DENSITY-LIPOPROTEIN", Journal of pathology, 179(3), 1996, pp. 294-302

Abstract

This study has demonstrated the toxicity to human monocyte-macrophages of low-density lipoprotein (LDL) which had been artificially oxidized using copper sulphate, The assays of cell damage used were tritiatedadenine release, neutral red staining, lactate dehydrogenase leakage,and MTT dye reduction, Toxicity was concentration- and time-dependent, Exposure to native LDL under the same conditions did not result in toxicity. Transmission electron microscopy of cells exposed to oxidizedLDL showed characteristic changes of apoptosis, including chromatin condensation and a decrease in cell volume, There was extensive loss ofcell surface protrusions and evidence of the phagocytosis of apoptotic cells by neighbouring monocyte-macrophages. Apoptotic features preceded the increased membrane permeability revealed by the release of radioactivity from cells preloaded with tritiated adenine and by lactate dehydrogenase leakage, DNA fragmentation was indicated by nick end-labelling using the terminal transferase enzyme (TUNEL), The number of TUNEL-positive cells was markedly greater in cells exposed to oxidized LDL, compared with those incubated as no-additions controls, Inhibitionof ne novo protein synthesis with cycloheximide and of Ca2+/Mg2+-activated endonuclease activity with aurintricarboxylic acid or zinc ion did not inhibit the toxicity produced by oxidized LDL.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 29/02/20 alle ore 02:29:23