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Titolo:
CHARACTERIZATION OF ELASTASE-DEFICIENT CLINICAL ISOLATES OF PSEUDOMONAS-AERUGINOSA
Autore:
HAMOOD AN; GRISWOLD J; COLMER J;
Indirizzi:
TEXAS TECH UNIV,HLTH SCI CTR,DEPT MICROBIOL & IMMUNOL LUBBOCK TX 79430 TEXAS TECH UNIV,HLTH SCI CTR,DEPT SURG LUBBOCK TX 79430
Titolo Testata:
Infection and immunity
fascicolo: 8, volume: 64, anno: 1996,
pagine: 3154 - 3160
SICI:
0019-9567(1996)64:8<3154:COECIO>2.0.ZU;2-U
Fonte:
ISI
Lingua:
ENG
Soggetto:
EXOTOXIN-A EXPRESSION; HOST-RANGE VECTORS; ESCHERICHIA-COLI; TOXIN-A; TRANSCRIPTIONAL ACTIVATOR; VIRULENCE GENES; MESSENGER-RNA; CLONING; LASA; MUTANTS;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Science Citation Index Expanded
Citazioni:
59
Recensione:
Indirizzi per estratti:
Citazione:
A.N. Hamood et al., "CHARACTERIZATION OF ELASTASE-DEFICIENT CLINICAL ISOLATES OF PSEUDOMONAS-AERUGINOSA", Infection and immunity, 64(8), 1996, pp. 3154-3160

Abstract

Elastase production in Pseudomonas aeruginosa is regulated by the lasR, lasI, rhlR, and rhlI genes. Recently we have analyzed several clinical isolates of P. aeruginosa for the production of elastase and otherextracellular virulence factors. Four of these isolates (CIT1, CIW5, CIW7, and CIW8) produced no elastolytic activity. We have characterized these isolates with respect to their elastase-deficient phenotype. Elastase was detected by immunoblotting experiments using elastase-specific antiserum. We also determined the presence of lasB and lasR mRNAsby Northern (RNA) blot hybridization experiments using lasB and lasR internal probes, respectively. None of the four elastase-deficient strains produced either the elastase protein or the lasB mRNA. Complementation experiments (using plasmids carrying either the lasB or the lasRgene) were conducted to determine if the isolates carry defective lasB or lasR genes. The presence of either a lasB or a lasR plasmid in CIW7 and CIW8 resulted in the production of very low levels of elastase and lasB mRNA. Neither elastase nor lasB mRNA was detected in CIT1 andCIW5 carrying the lasB plasmid. The presence of the lasR plasmid in CIT1 and CIW5 resulted in the production of lasB mRNA and elastase protein in CIW5 only. All elastase-deficient strains produced detectable levels of lasR mRNA which were enhanced in the presence of the lasR plasmid. The Pseudomonas autoinducer (which is encoded by lasI) was also produced by all strains. CIT1 produced both hemolysin and alkaline protease but was defective in pyocyanin production. These results suggestthat (i) CIT1 may contain a defect in a lasB-regulatory gene, (ii) CIW5 carries a defect within lasR, and (iii) the defect in isolates CIW7and CIW8 affects the efficiency of lasB transcription.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 27/11/20 alle ore 11:54:31