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Titolo:
EPIDERMAL GROWTH FACTOR-BINDING PROTEIN IN MYCOBACTERIUM-AVIUM AND MYCOBACTERIUM-TUBERCULOSIS - A POSSIBLE ROLE IN THE MECHANISM OF INFECTION
Autore:
BERMUDEZ LE; PETROFSKY M; SHELTON K;
Indirizzi:
CALIF PACIFIC MED CTR,RES INST,KUZELL INST ARTHRIT & INFECT DIS,2200 WEBSTER RD,ROOM 305 SAN FRANCISCO CA 94115
Titolo Testata:
Infection and immunity
fascicolo: 8, volume: 64, anno: 1996,
pagine: 2917 - 2922
SICI:
0019-9567(1996)64:8<2917:EGFPIM>2.0.ZU;2-M
Fonte:
ISI
Lingua:
ENG
Soggetto:
GLYCERALDEHYDE-3-PHOSPHATE DEHYDROGENASE; ESCHERICHIA-COLI; STAPHYLOCOCCUS-AUREUS; HUMAN MACROPHAGES; PLASMIN RECEPTOR; SKELETAL-MUSCLE; COMPLEX; FIBRONECTIN; SCHISTOSOMIASIS; INTERLEUKIN-2;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Science Citation Index Expanded
Citazioni:
33
Recensione:
Indirizzi per estratti:
Citazione:
L.E. Bermudez et al., "EPIDERMAL GROWTH FACTOR-BINDING PROTEIN IN MYCOBACTERIUM-AVIUM AND MYCOBACTERIUM-TUBERCULOSIS - A POSSIBLE ROLE IN THE MECHANISM OF INFECTION", Infection and immunity, 64(8), 1996, pp. 2917-2922

Abstract

Epidermal growth factor (EGF) is a potent mitogen for a variety of eukaryotic cells, EGF is found in a number of tissues and is prevalent in necrotic tissues and granulomata. The biological effect of EGF on mammalian cells is initiated by the binding to a specific receptor. BothMycobacterium avium and Mycobacterium tuberculosis cause lung infections and localized or disseminated disease in both patients without AIDS and those with AIDS. Histopathologic studies show necrosis in the lung, liver, and splenic tissues of patients with disseminated mycobacterial infection. In the course of experiments to examine the effect of growth factors on macrophages, it was observed that M. avium and M. tuberculosis but not Mycobacterium smegmatis cultured in the presence of5, 50, or 500 ng of EGF per ml grew significantly faster than mycobacteria cultured in the absence of EGF. I-125-EGF was found to bind to M. avium and M. tuberculosis, and the binding was competitively inhibited by unlabeled EGF. A receptor for EGF was identified on mycobacteria. Incubation of mycobacteria with EGF prior to infection of macrophagemonolayers resulted in faster bacterial growth within macrophages compared with that of mycobacteria not incubated with EGF. EGF-binding protein was cloned and expressed in Escherichia coli, and subsequently the protein was purified and the N-terminal amino acids were sequenced. These results suggest that EGF is a growth factor for pathogenic mycobacteria in granulomatous tissues and within macrophages and might enhance growth rates of both intracellular and extracellular mycobacteriain the site of infection.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 24/10/20 alle ore 11:13:45