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Titolo:
EXPRESSION OF LA45 REACTIVE BETA(2)-MICROGLOBULIN FREE HLA CLASS-I ALPHA-CHAINS ON ACTIVATED T-CELLS IS REGULATED BY INTERNALIZATION, CONSTITUTIVE AND PROTEIN-KINASE-C INDUCIBLE RELEASE
Autore:
PICKL WF; HOLTER W; STOCKL J; MAJDIC O; KNAPP W;
Indirizzi:
UNIV VIENNA,INST IMMUNOL,BORSCHKLEGASSE 8A A-1090 VIENNA AUSTRIA
Titolo Testata:
Tissue antigens
fascicolo: 1, volume: 48, anno: 1996,
pagine: 15 - 21
SICI:
0001-2815(1996)48:1<15:EOLRBF>2.0.ZU;2-P
Fonte:
ISI
Lingua:
ENG
Soggetto:
HEAVY-CHAINS; MONOCLONAL-ANTIBODIES; SURFACE ANTIGENS; LEUKEMIA-CELLS; LYMPHOCYTES-T; MOLECULES; BETA-2-MICROGLOBULIN; METALLOPROTEASE; RESTRICTION; ENDOCYTOSIS;
Keywords:
ACTIVATION ANTIGEN; HLA CLASS I ALPHA-CHAINS; METALLOPROTEASE; MODULATION; 1,10-PHENANTROLINE; PKC; PMA;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Science Citation Index Expanded
Citazioni:
39
Recensione:
Indirizzi per estratti:
Citazione:
W.F. Pickl et al., "EXPRESSION OF LA45 REACTIVE BETA(2)-MICROGLOBULIN FREE HLA CLASS-I ALPHA-CHAINS ON ACTIVATED T-CELLS IS REGULATED BY INTERNALIZATION, CONSTITUTIVE AND PROTEIN-KINASE-C INDUCIBLE RELEASE", Tissue antigens, 48(1), 1996, pp. 15-21

Abstract

HLA Class I molecules on activated T cells are expressed as mAb W6/32reactive heterodimers associated with beta(2)-microglobulin (beta(2)-m) and also as mAb LA45 reactive beta(2)-m free HLA Class I alpha-chains. However, the regulation of free alpha-chain expression remained enigmatic. Here we show, that the amount of cell surface expressed free heavy chains is influenced by two distinct mechanisms. Firstly, a proportion of expressed molecules are cleaved and give rise to a soluble pool of HLA Class T molecules. We provide evidence that, besides the previously described constitutive release of free alpha chains, a secondphorbol ester inducible release mechanism involving activation of protein kinase C (PKC) does exist. We demonstrate that both the constitutive and the enhanced release of LA45 reactive HLA Class I alpha-chainsare the consequence of a cell membrane bound proteolytic activity with the characteristics of a 1, 10 phenanthroline sensitive metalloprotease. Secondly, we report that a distinct fraction of mAb tagged free alpha-chains is internalized via an n-ethylmaleimide Sensitive pathway. Together this data suggests that the expression of free alpha-chains is regulated by pathways governing release and internalization.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 28/09/20 alle ore 05:49:46