Catalogo Articoli (Spogli Riviste)

OPAC HELP

Titolo:
OPTICAL IMAGING OF RESPIRATORY BURST ACTIVITY IN NEWBORN RAT MEDULLARY BLOCK PREPARATIONS
Autore:
ONIMARU H; KANAMARU A; HOMMA I;
Indirizzi:
SHOWA UNIV,SCH MED,DEPT PHYSIOL,SHINAGAWA KU,1-5-8 HATANODAI TOKYO 142 JAPAN
Titolo Testata:
Neuroscience research
fascicolo: 2, volume: 25, anno: 1996,
pagine: 183 - 190
SICI:
0168-0102(1996)25:2<183:OIORBA>2.0.ZU;2-5
Fonte:
ISI
Lingua:
ENG
Soggetto:
SPINAL-CORD PREPARATION; ROSTRAL VENTROLATERAL MEDULLA; RHYTHM GENERATION; NEURONS; CONNECTIONS; RECORDINGS; INVITRO; MAMMALS; SLICES;
Keywords:
RESPIRATORY CENTER; OPTICAL IMAGING; VENTROLATERAL MEDULLA; NUCLEUS TRACTUS SOLITARIUS; INTERMEDIATE RETICULAR ZONE; NEWBORN RAT;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Citazioni:
46
Recensione:
Indirizzi per estratti:
Citazione:
H. Onimaru et al., "OPTICAL IMAGING OF RESPIRATORY BURST ACTIVITY IN NEWBORN RAT MEDULLARY BLOCK PREPARATIONS", Neuroscience research, 25(2), 1996, pp. 183-190

Abstract

We report on the optical imaging of excitation propagation induced byelectrical stimulation of the nucleus tractus solitarius (NTS) area and subsequent inspiratory burst activity in the ventrolateral medulla (VLM) of a medullary block preparation. A medullary block preparation with a thickness of 1.0-1.4 mm was made from brainstems isolated from 0- to 4-day-old rats and stained with a fluorescent voltage-sensitive dye, RH795. Neuronal responses in the VLM evoked by electrical stimulation were recorded as a fluorescence change using an optical recordingapparatus with a 128 x 128 photodiode array and a maximum time resolution of 0.6 ms. Motoneuronal activity was simultaneously recorded at the contralateral hypoglossal nerve roots. Neuronal excitation evoked by stimulation of the NTS area propagated to the VLM through the intermediate reticular zone (IRt). In contrast, caudal VLM stimulation induced excitation which propagated to the rostral VLM without any detectable excitation propagation in the IRt toward the NTS area from the VLM. NTS stimulation also induced an inspiratory burst activity in the hypoglossal nerve root activity with a 150-200 ms delay. Fluorescence changes corresponding to the inspiratory burst activity were observed in the VLM which coincided with the area in which the localization of many respiratory neurons had been demonstrated in previous studies using whole-brainstem preparations. The present results show the feasibilityof using optical recordings for the analysis of respiratory neuron activity as well as for analysis of the transmission pathway of afferentand/or efferent information in the medulla.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 25/11/20 alle ore 04:09:47