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Titolo:
MASS-SPECTROMETRIC CHARACTERIZATION OF SEQUENCE-SPECIFIC COMPLEXES OFDNA AND TRANSCRIPTION FACTOR PU.1 DNA-BINDING DOMAIN
Autore:
CHENG XH; MORIN PE; HARMS AC; BRUCE JE; BENDAVID Y; SMITH RD;
Indirizzi:
ABBOTT LABS,DIV PHARMACEUT DISCOVERY N CHICAGO IL 60064 BATTELLE MEM INST,PACIFIC NW LABS,ENVIRONM MOL SCI LAB RICHLAND WA 99352 UNIV TORONTO,DEPT MED BIOPHYS TORONTO ON M5G 2M9 CANADA ONTARIO CANC INST TORONTO ON M5G 2M9 CANADA UNIV TORONTO,SUNNY BROOK MED SCI CTR,DEPT CANC RES TORONTO ON M4N 3M5CANADA
Titolo Testata:
Analytical biochemistry
fascicolo: 1, volume: 239, anno: 1996,
pagine: 35 - 40
SICI:
0003-2697(1996)239:1<35:MCOSCO>2.0.ZU;2-I
Fonte:
ISI
Lingua:
ENG
Soggetto:
ELECTROSPRAY-IONIZATION; OLIGONUCLEOTIDE DUPLEX; BIOMOLECULES; PRINCIPLES; PROTEINS;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Citazioni:
22
Recensione:
Indirizzi per estratti:
Citazione:
X.H. Cheng et al., "MASS-SPECTROMETRIC CHARACTERIZATION OF SEQUENCE-SPECIFIC COMPLEXES OFDNA AND TRANSCRIPTION FACTOR PU.1 DNA-BINDING DOMAIN", Analytical biochemistry, 239(1), 1996, pp. 35-40

Abstract

Electrospray ionization mass spectrometry (ESI-MS) has been used to study the noncovalent interaction of the 13.5-kDa DNA binding domain ofPU.1 (PU.1-DBD) with specific double-stranded DNA (dsDNA) target molecules. Mixtures of PU.1-DBD protein and wildtype target DNA sequence yielded ESI-MS spectra showing only protein-dsDNA complex ions of 1:1 stoichiometry and free dsDNA. When PU.1-DBD protein, wild type target DNA, and a mutant target DNA lacking the consensus sequence were mixed,only the 1:1 complex with the wild-type DNA was observed, consistent with gel electrophoresis mobility shift assay results, demonstrating the observation of sequence-specific protein-dsDNA complexes using ESI-MS. (C) 1996 Academic Press, Inc.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 08/07/20 alle ore 05:28:11