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Titolo:
A CHARACTERISTIC BENT CONFORMATION OF RNA PSEUDOKNOTS PROMOTES -1 FRAMESHIFTING DURING TRANSLATION OF RETROVIRAL RNA
Autore:
CHEN XY; KANG HS; SHEN LX; CHAMORRO M; VARMUS HE; TINOCO I;
Indirizzi:
UNIV CALIF BERKELEY,DEPT CHEM BERKELEY CA 94720 UNIV CALIF BERKELEY,DEPT CHEM BERKELEY CA 94720 UNIV CALIF BERKELEY,LAWRENCE BERKELEY LAB,DIV BIOL STRUCT BERKELEY CA94720 SRI INT,DIV LIFE SCI MENLO PK CA 94025 UNIV CALIF SANTA CRUZ,SINSHEIMER LABS SANTA CRUZ CA 95064 NCI,NIH BETHESDA MD 20892
Titolo Testata:
Journal of Molecular Biology
fascicolo: 4, volume: 260, anno: 1996,
pagine: 479 - 483
SICI:
0022-2836(1996)260:4<479:ACBCOR>2.0.ZU;2-7
Fonte:
ISI
Lingua:
ENG
Soggetto:
IDENTIFICATION; SIGNAL; VIRUS;
Keywords:
RNA; PSEUDOKNOT; NMR; FRAMESHIFTING; RETROVIRUSES;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Citazioni:
14
Recensione:
Indirizzi per estratti:
Citazione:
X.Y. Chen et al., "A CHARACTERISTIC BENT CONFORMATION OF RNA PSEUDOKNOTS PROMOTES -1 FRAMESHIFTING DURING TRANSLATION OF RETROVIRAL RNA", Journal of Molecular Biology, 260(4), 1996, pp. 479-483

Abstract

The structures of four different RNA pseudoknots that provide one of the signals required for ribosomal frameshifting in mouse mammary tumor virus have been determined by NMR. The RNA pseudoknots have similar sequences and assume similar secondary structures, but show significantly different frameshifting efficiencies. The three-dimensional structures of one frameshifting and one non-frameshifting RNA pseudoknot hadbeen determined previously by our group. Here we determine the structures of two new RNA pseudoknots, and relate the structures of all fourpseudoknots to their frameshifting abilities. The two efficient frameshifting pseudoknots adopt characteristic bent conformations with stem1 bending towards the major groove of stem 2. In contrast, the two poor frameshifting pseudoknots have structures very different from each other and from the efficient frameshifters. One has linear, coaxially stacked stems, the other has stems twisted and bent, but in the opposite direction to the efficient frameshifters. Changes in loop size thatfavor bending (shorter loops) increase frameshifting efficiency; longer loops that allow linear arrangement of the stems decrease frameshifting. Frameshifting pseudoknots in feline immunodeficiency virus and simian retrovirus have different loop sequences, but the sequences at their stem junctions imply the same bent conformation as in the mouse mammary tumor viral RNA. The requirement for a precise pseudoknot conformation for efficient frameshifting strongly implies that a specific interaction occurs between the viral RNA pseudoknot and the host protein-synthesizing machinery. (C) 1996 Academic Press Limited

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 29/11/20 alle ore 04:51:35