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Titolo:
A NOVEL ENZYME-SYSTEM FOR THE REDUCTION OF 3-OXO BILE-ACIDS IN HUMAN RED-BLOOD-CELLS
Autore:
GOTO J; MIURA H; ANDO N; YAMATO Y; IKEGAWA S; NAMBARA T; MAKINO I;
Indirizzi:
TOHOKU UNIV,FAC PHARMACEUT SCI SENDAI MIYAGI 98077 JAPAN ASAHIKAWA MED COLL,DEPT INTERNAL MED 2 ASAHIKAWA HOKKAIDO 078 JAPAN
Titolo Testata:
Steroids
fascicolo: 7, volume: 61, anno: 1996,
pagine: 416 - 420
SICI:
0039-128X(1996)61:7<416:ANEFTR>2.0.ZU;2-1
Fonte:
ISI
Lingua:
ENG
Soggetto:
CHROMATOGRAPHY MASS-SPECTROMETRY; CHEMICAL IONIZATION DETECTION; STEROIDS; METABOLISM; LIVER;
Keywords:
3-OXO BILE ACID; ENZYMATIC REDUCTION; EXTRAHEPATIC BIOTRANSFORMATION; RED BLOOD CELL; GAS CHROMATOGRAPHY MASS SPECTROMETRY; ION CLUSTER TECHNIQUE;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Science Citation Index Expanded
Citazioni:
9
Recensione:
Indirizzi per estratti:
Citazione:
J. Goto et al., "A NOVEL ENZYME-SYSTEM FOR THE REDUCTION OF 3-OXO BILE-ACIDS IN HUMAN RED-BLOOD-CELLS", Steroids, 61(7), 1996, pp. 416-420

Abstract

7 alpha,12 alpha-Dihydroxy-3-oxo- and 3,7,12-trioxo-5 beta-cholanoic acids labeled with O-18 atoms were incubated with human red blood cells, and the biotransformation products were separated and characterizedby gas chromatography-mass spectrometry as the pentafluorobenzyl ester-trimethylsilyl and -dimethylethylsilyl ether derivatives with the negative ion chemical ionization mode. The reduced products, 3 beta,7 alpha-12 alpha-trihydroxy-5 beta-cholanoic acid for the former, and 3 alpha-hydroxylated dioxo bile acid together with 3 beta-hydroxylated 7,12-dioxo-5 beta-cholanoic acid for the latter, were identified as metabolites. When 3-oxo bile acid was incubated with human blood denatured at 70 degrees C for 2 min, no metabolites were formed. The enzymic reduction activity has been localized in the red blood cell fraction.

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Documento generato il 15/07/20 alle ore 13:38:24