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Titolo:
PARATHYROID HORMONE-RELATED PROTEIN (PTHRP) IS AN EPIDERMAL GROWTH FACTOR-REGULATED SECRETORY PRODUCT OF HUMAN PROSTATIC EPITHELIAL-CELLS
Autore:
CRAMER SD; PEEHL DM; EDGAR MG; WONG ST; DEFTOS LJ; FELDMAN D;
Indirizzi:
STANFORD UNIV,SCH MED,DEPT UROL STANFORD CA 94305 STANFORD UNIV,SCH MED,DEPT MED STANFORD CA 94305 UNIV CALIF SAN DIEGO,DEPT MED SAN DIEGO CA 92103 VET ADM MED CTR SAN DIEGO CA 00000
Titolo Testata:
The Prostate
fascicolo: 1, volume: 29, anno: 1996,
pagine: 20 - 29
SICI:
0270-4137(1996)29:1<20:PHP(IA>2.0.ZU;2-2
Fonte:
ISI
Lingua:
ENG
Soggetto:
MESSENGER RIBONUCLEIC-ACIDS; NORMAL HUMAN KERATINOCYTES; OSTEOBLAST-LIKE CELLS; GC-RICH PROMOTER; PEPTIDE GENE; VITAMIN-D; RAT-TISSUES; IN-VITRO; EXPRESSION; RECEPTOR;
Keywords:
IMMUNOHISTOCHEMISTRY; RADIOIMMUNOASSAY; NORTHERN ANALYSIS; 1,25-DIHYDROXYVITAMIN D-3;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Science Citation Index Expanded
Citazioni:
53
Recensione:
Indirizzi per estratti:
Citazione:
S.D. Cramer et al., "PARATHYROID HORMONE-RELATED PROTEIN (PTHRP) IS AN EPIDERMAL GROWTH FACTOR-REGULATED SECRETORY PRODUCT OF HUMAN PROSTATIC EPITHELIAL-CELLS", The Prostate, 29(1), 1996, pp. 20-29

Abstract

Parathyroid hormone-related protein (PTHrP) has previously been shownto be expressed in human prostatic tissue and in prostatic cancer cell lines. In the present study, PTHrP immunoreactivity was detected in the glandular epithelium of normal prostate and benign prostatic hyperplasia (BPH), as well as in prostatic adenocarcinoma (CaP). Epithelialcell cultures derived from normal, BPH, and CaP tissues were also stained by antibodies against PTHrP, and northern analysis revealed multiple transcripts of PTHrP in the cellular RNA. PTHrP (1-34) was measurable by radioimmunoassay (RIA) in media conditioned by the prostatic epithelial cell cultures, and PTHrP accumulated in conditioned media during a 72 hr time course, Addition of complete growth medium to starvedcells resulted in increased PTHrP mRNA levels by 1 hr, with maximal stimulation at 8-24 kr. Several individual factors contained in the complete growth medium were tested for their ability to regulate PTHrP expression. Epidermal growth factor (EGF) was the major inducer of PTHrPexpression, while cholera toxin, bovine pituitary extract, hydrocortisone, and insulin had minimal or no effect on PTHrP transcript levels,Since each of these factors is growth stimulatory, the unique abilityof EGF to induce PTHrP is apparently unrelated to mitogenicity, 1,25-Dihydroxyvitamin D-3 [1,25(OH)(2)D-3], an inhibitor of PTHrP expression in several other cell types, had no effect on steady-state levels ofPTHrP mRNA expressed by epithelial cells in complete growth medium, although prostate cells have vitamin D receptors and are responsive to 1,25(OH)(2)D-3 in other ways. Our results indicate that PTHrP expression is not confined to the neuroendocrine cells of the human prostate and that our culture system can be used as a model to investigate the role of PTHrP in the prostate. (C) 1996 Wiley-Liss, Inc.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 02/04/20 alle ore 19:01:30