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Titolo:
ASSEMBLY OF VIRUS-LIKE PARTICLES IN INSECT CELLS INFECTED WITH A BACULOVIRUS CONTAINING A MODIFIED COAT PROTEIN GENE OF POTATO LEAFROLL LUTEOVIRUS
Autore:
LAMB JW; DUNCAN GH; REAVY B; GILDOW FE; MAYO MA; HAY RT;
Indirizzi:
SCOTTISH CROP RES INST DUNDEE DD2 5DA SCOTLAND SCOTTISH CROP RES INST DUNDEE DD2 5DA SCOTLAND UNIV ST ANDREWS,SCH MED & BIOMED SCI ST ANDREWS KY16 9AL FIFE SCOTLAND PENN STATE UNIV,DEPT PLANT PATHOL UNIVERSITY PK PA 16803
Titolo Testata:
Journal of General Virology
, volume: 77, anno: 1996,
parte:, 7
pagine: 1349 - 1358
SICI:
0022-1317(1996)77:<1349:AOVPII>2.0.ZU;2-2
Fonte:
ISI
Lingua:
ENG
Soggetto:
WESTERN YELLOWS VIRUS; APHID-TRANSMISSIBILITY; NUCLEOTIDE-SEQUENCE; DWARF VIRUS; EXPRESSION; RNA; PURIFICATION; READTHROUGH; EPITOPES;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Science Citation Index Expanded
Citazioni:
42
Recensione:
Indirizzi per estratti:
Citazione:
J.W. Lamb et al., "ASSEMBLY OF VIRUS-LIKE PARTICLES IN INSECT CELLS INFECTED WITH A BACULOVIRUS CONTAINING A MODIFIED COAT PROTEIN GENE OF POTATO LEAFROLL LUTEOVIRUS", Journal of General Virology, 77, 1996, pp. 1349-1358

Abstract

DNA encoding the coat protein (P3) of a Scottish isolate of potato leafroll virus (PLRV) was inserted into the genome of Autographa californica nucleopolyhedrovirus (AcNPV) such that the coat protein was expressed either in an unmodified form or with the addition of the amino acid sequence MHHHHHHGDDDDKDAMG at the N terminus (P3-6H), Insect cells infected with these recombinant baculoviruses accumulated substantial amounts of P3 and P3-6H. P3 could not be recovered from cell extracts unless it was denatured in SDS but a proportion of the P3-6H was recoverable in a soluble form in nondenaturing conditions, Immunogold labelling of sections of infected cells showed that P3 accumulated in nuclei in large amorphous bodies, In contrast, although much of the P3-6H also accumulated in nuclei, it formed virus-like particles (VLP) which were often grouped in close-packed, almost crystalline arrays, When electron microscope grids coated with antibodies to PLRV were floated oncell extracts containing P3-6H, VLP were trapped which were indistinguishable from PLRV particles trapped from extracts of PLRV-infected plants, The VLP co-sed imented in sucrose gradients with PLRV particles which suggests that the VLP contained RNA, VLP collected from sucrose density gradient fractions contained protein which reacted with nickelchelated to nitrilotriacetic acid, a histidine-specific reagent. Cells infected with either recombinant baculovirus also synthesized a protein, with an M(r) of about 17000, which was shown to be the translation product of the P4 gene which is in the +1 reading frame within the coat protein gene, This protein was also found in the nuclear fraction of infected cells but was more readily soluble than was P3.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 09/08/20 alle ore 23:11:56